FAM50A Polyclonal antibody

FAM50A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19849-1-AP

Synonyms

Protein HXC-26, Protein HXC 26, Protein FAM50A, HXC26, HXC 26

---

Validation Data Gallery

• 

  WB analysis using 19849-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19849-1-AP (FAM50A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat colon using 19849-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 19849-1-AP (FAM50A antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 19849-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 19849-1-AP (FAM50A antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, Jurkat cells, NIH/3T3 cells
• Positive IHC detected in: rat colon tissue, rat kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19849-1-AP targets FAM50A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAM50A fusion protein Ag13907
• Full Name: family with sequence similarity 50, member A
• Calculated Molecular Weight: 325 aa, 39 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC000028
• Gene Symbol: FAM50A
• Gene ID (NCBI): 9130
• RRID: AB_10641032
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FAM50A belongs to the FAM50 family. It is highly conserved in length and sequence across different species. It is a basic protein containing a nuclear localization signal, and may function as a DNA-binding protein or a transcriptional factor.