Tested Applications
| Positive WB detected in | C2C12 cells, HEK-293 cells, rat brain tissue, mouse brain, mouse cerebellum |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | mouse brain tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21537-1-AP targets FAM134B in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16176 Product name: Recombinant human FAM134B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 282-455 aa of BC030517 Sequence: ELDFSALCPKISLTVAAKELSVSDTDVSEVSWTDNGTFNLSEGYTPQTDTSDDLDRPSEEVFSRDLSDFPSLENGMGTNDEDELSLGLPTELKRKKEQLDSGHRPSKETQSAAGLTLPLNSDQTFHLMSNLAGDVITAAVTAAIKDQLEGVQQALSQAAPIPEEDTDTEEGDDF Predict reactive species |
| Full Name | family with sequence similarity 134, member B |
| Calculated Molecular Weight | 497 aa, 55 kDa |
| Observed Molecular Weight | 65 kDa |
| GenBank Accession Number | BC030517 |
| Gene Symbol | FAM134B |
| Gene ID (NCBI) | 54463 |
| RRID | AB_2878879 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H6L5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FAM134B, also known as the reticulophagy regulator 1 (RETREG1) or JK-1, is a member of the family with sequence similarity 134. FAM134B is an important ER-phagy receptor. FAM134B regulates the size and shape of the ER and participates in some ER-phagy-related processes(PMID: 33199694). FAM134B inhibition contributes to impair proteostasis in the ER due to the accumulation of misfolded or aggregated proteins, which in turn leads to compromised neuronal survival and progressive neuronal degenerative diseases. In addition, FAM134B mutations are common in patients with colorectal adenocarcinoma, and oesophageal squamous cell carcinoma(PMID: 29226326).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for FAM134B antibody 21537-1-AP | Download protocol |
| IP protocol for FAM134B antibody 21537-1-AP | Download protocol |
| WB protocol for FAM134B antibody 21537-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-FAM134B (RETREG1) antibody (Cat# 21537-1-AP) has accumulated 79 citations across high-impact journals including Nature, Cell Research, Nature Cell Biology, Autophagy, Nature Communications, Molecular Cell, Developmental Cell, EMBO Journal, PNAS, and eLife. Citations span research fields such as selective ER-phagy/reticulophagy, endoplasmic reticulum stress, autophagy, cancer (hepatocellular carcinoma, melanoma, glioblastoma), neurodegeneration (Parkinson's disease), viral infections (SARS-CoV-2, Dengue, Zika), cardiac fibrosis, diabetic nephropathy, and protein quality control.
| Species | Application | Title |
|---|---|---|
Nature Heteromeric clusters of ubiquitinated ER-shaping proteins drive ER-phagy
| ||
Cell Res The STX17-SNAP47-VAMP7/VAMP8 complex is the default SNARE complex mediating autophagosome-lysosome fusion | ||
Nat Cell Biol Cholesterol sensing by the SCAP-FAM134B complex regulates ER-phagy and STING innate immunity. | ||
Autophagy Mass spectrometry proteomics reveals a function for mammalian CALCOCO1 in MTOR-regulated selective autophagy. |
Reviews
What customers say
One reviewer (H.A.) used this antibody for Western Blot on HEK293T cells at a 1:1000 dilution and rated it 4 out of 5 stars. They reported that the antibody works well overall, though they observed a few non-specific bands in HEK293T lysates. The review was posted in April 2020.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
H (Verified Customer) (04-06-2020) | The antibody works well. There were a few non-specific bands in HEK293T lysates though.
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