FAM127A Polyclonal antibody

FAM127A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 10307-1-AP

Synonyms

CAAX box protein 1, CXX1, FAM127A, Mar8, MAR8C, Mart8, MART8C, Protein FAM127A

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Validation Data Gallery

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  WB analysis of human brain using 10307-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10307-1-AP (FAM127A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 10307-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10307-1-AP (FAM127A antibody) at dilution of 1:100 (under 25x lens).

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Tested Applications

• Positive WB detected in: human brain tissue
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

10307-1-AP targets FAM127A in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAM127A fusion protein Ag0298
• Full Name: family with sequence similarity 127, member A
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC002410
• Gene Symbol: FAM127A
• Gene ID (NCBI): 8933
• RRID: AB_2101197
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CAAX box protein 1 is also name as CXX1 or FAM127A. The function of CXX1 remains largely unknown. Catalog#10307-1-AP is a rabbit polyclonal antibody raised against the full-length of human CXX1. Two FAM127A proteins are presented in UniProt: O15255 and A6ZKI3 (UniProt Number). This antibody specially recognises the O15255.

Publications

Species	Application	Title
mouse	WB	J Cell Sci; The PRDM14-CtBP1/2-PRC2 complex regulates transcriptional repression during the transition from primed to naïve pluripotency.; Authors - Maiko Yamamoto