FAM125A Polyclonal antibody

FAM125A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25816-1-AP

Synonyms

CFBP, ESCRT I complex subunit MVB12A, FAM125A, Protein FAM125A

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Validation Data Gallery

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  WB analysis of Jurkat using 25816-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25816-1-AP (FAM125A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 25816-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 25816-1-AP (FAM125A Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 25816-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 25816-1-AP (FAM125A Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 25816-1-AP

  Immunofluorescent analysis of HeLa cells using 25816-1-AP (FAM125A antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IHC detected in: human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25816-1-AP is FAM125A Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAM125A fusion protein Ag22919
• Full Name: family with sequence similarity 125, member A
• Calculated Molecular Weight: 273 aa, 29 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC007883
• Gene Symbol: FAM125A
• Gene ID (NCBI): 93343
• RRID: AB_2880250
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FAM125A, also named as MVB12A and CFBP, is a component of the ESCRT-I complex. FAM125A is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies. FAM125A has three isoforms with MW 27-28 kDa and 25 kDa.