FAM125A Polyclonal antibody

FAM125A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25816-1-AP

Synonyms

CFBP, ESCRT I complex subunit MVB12A, FAM125A, Protein FAM125A



Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25816-1-AP is FAM125A Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAM125A fusion protein Ag22919
Full Name family with sequence similarity 125, member A
Calculated Molecular Weight 273 aa, 29 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC007883
Gene Symbol FAM125A
Gene ID (NCBI) 93343
RRIDAB_2880250
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FAM125A, also named as MVB12A and CFBP, is a component of the ESCRT-I complex. FAM125A is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies. FAM125A has three isoforms with MW 27-28 kDa and 25 kDa.

Protocols

Product Specific Protocols
WB protocol for FAM125A antibody 25816-1-APDownload protocol
IHC protocol for FAM125A antibody 25816-1-APDownload protocol
IF protocol for FAM125A antibody 25816-1-APDownload protocol
Standard Protocols
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