ABHD17A Polyclonal antibody

ABHD17A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 15854-1-AP

Synonyms

FAM108A1, EC:3.1.2.22, C19orf27, Alpha/beta hydrolase domain-containing protein 17A, Abhydrolase domain-containing protein 17A

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Validation Data Gallery

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  WB analysis using 15854-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15854-1-AP (ABHD17A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 15854-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15854-1-AP (ABHD17A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 15854-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15854-1-AP (ABHD17A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: PC-3 cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

15854-1-AP targets ABHD17A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ABHD17A fusion protein Ag8697
• Full Name: family with sequence similarity 108, member A1
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC000158
• Gene Symbol: ABHD17A
• Gene ID (NCBI): 81926
• RRID: AB_2878193
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Alpha/beta hydrolase domain-containing protein 17A (ABHD17A) is a membrane-localized thioesterase, which regulates H- and N-RAS39, also catalyzes the depalmitoylation of CNAβ1 causing it to redistribute from the PM to the cytosol and the Golgi (PMID: 34663815). ABHD17a is a major acyl protein thioesterase that site-specifically deacylates the STREX domain of the BK channel to control channel activity (PMID: 32913120). Western blot analysis detected ABHD17A at an apparent molecular mass of 34 kDa (PMID: 27307232), and the 70 kDa-band could be the dimer.

Publications

Species	Application	Title
		J Neurosci; Identification of PSD-95 Depalmitoylating Enzymes.; Authors - Norihiko Yokoi
	WB	J Cell Sci; Activity-dependent post-translational regulation of palmitoylating and depalmitoylating enzymes in the hippocampus; Authors - Danya Abazari
		J Biol Chem; The Tumor Suppressor NF2 Modulates TEAD4 Stability and Activity in Hippo Signaling via Direct Interaction; Authors - Mengying Wu
mouse	WB	Sci Adv; Atf3-mediated metabolic reprogramming in hepatic macrophage orchestrates metabolic dysfunction-associated steatohepatitis; Authors - Shuwei Hu