FAF1 Polyclonal antibody

• Featured Product
• KD/KO Validated

FAF1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 10271-1-AP

Synonyms

CGI 03, FAF1, FAS associated factor 1, hFAF1, HFAF1s, UBXD12, UBXN3A

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Validation Data Gallery

• 

  WB analysis of HeLa using 10271-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10271-1-AP (FAF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10271-1-AP

  WB result of FAF1 antibody (10271-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAF1 transfected HEK-293 cells.

• 

  WB analysis of mouse testis using 10271-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10271-1-AP (FAF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10271-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10271-1-AP (FAF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10271-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10271-1-AP (FAF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10271-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10271-1-AP (FAF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 10271-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10271-1-AP (FAF1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 10271-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10271-1-AP (FAF1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, mouse testis tissue, mouse brain tissue, K-562 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 5 publications below

Product Information

10271-1-AP targets FAF1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAF1 fusion protein Ag0407
• Full Name: Fas (TNFRSF6) associated factor 1
• Calculated Molecular Weight: 650aa, 74 kDa
• Observed Molecular Weight: 74 kDa
• GenBank Accession Number: BC004970
• Gene Symbol: FAF1
• Gene ID (NCBI): 11124
• RRID: AB_2100774
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FAF1 was detected most abundant in testis, slightly less abundant in skeletal muscle and heart, followed by prostate, thymus, ovary, small intestine, and colon, but not in the peripheral blood leukocytes.The N-terminal region (amino acid 1∼201) including the upstream ubiquitin homology domain of hFAF1 could bind with the death domain of Fas, which mediates programmed cell death, also called apoptosis, in a number of organ systems, notably the immune and nervous systems.

Publications

Species	Application	Title
human	WB,IF	Cell Death Differ; FAF1 mediates regulated necrosis through PARP1 activation upon oxidative stress leading to dopaminergic neurodegeneration.; Authors - Changsun Yu
human, mouse	WB,IHC,IF	Hum Mol Genet; Accumulation of the parkin substrate, FAF1, plays a key role in the dopaminergic neurodegeneration.; Authors - Sul Jee-Won JW
mouse	WB,IHC,IF	J Neurosci; Fas-associated factor 1 as a regulator of olfactory axon guidance.; Authors - Cheng Kai K
human	WB,IF	J Cell Sci; The p97-UBXN1 complex regulates aggresome formation.; Authors - Sirisha Mukkavalli ; KD Validated
human	WB	Cancer Manag Res; MiR-26a-5p Serves as an Oncogenic MicroRNA in Non-Small Cell Lung Cancer by Targeting FAF1.; Authors - Ming-Fan Ye ; KD Validated
mouse	IF	Neuroreport; Fas-associated factor 1 promotes in neurofibrillary tangle-mediated cell death of basal forebrain cholinergic neurons in P301L transgenic mice.; Authors - Jong Mu Kim

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