FABP7-Specific Polyclonal antibody

FABP7-Specific Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 14836-1-AP

Synonyms

FABP7, Brain-type fatty acid-binding protein, Brain lipid-binding protein, BLBP, B-FABP

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Validation Data Gallery

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  WB analysis of human brain using 14836-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14836-1-AP (FABP7-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-251 using 14836-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 14836-1-AP (FABP7-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 14836-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14836-1-AP (FABP7-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14836-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14836-1-AP (FABP7-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat brain using 14836-1-AP

  Immunofluorescent analysis of (-20°C Acetone) fixed rat brain tissue using FABP7-Specific antibody (14836-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of rat brain using 14836-1-AP

  Immunofluorescent analysis of (-20°C Acetone) fixed rat brain tissue using FABP7-Specific antibody (14836-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human brain tissue, U-251 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

14836-1-AP targets FABP7-Specific in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: fatty acid binding protein 7, brain
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 14-15 kDa
• GenBank Accession Number: NM_001446
• Gene Symbol: FABP7
• Gene ID (NCBI): 2173
• RRID: AB_2100458
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FABP7 (Fatty Acid Binding Protein-7), also known as B-FABP (Brain Fatty Acid-Binding Protein), is a member of FABP family which are intracellular proteins involved in lipid metabolism. Normally FABP7 is mainly expressed in brain and other neural tissues and is important in early nervous system development. In addition, FABP7 is considered a marker for some brain tumors and may have a role in tumorigenesis in the peripheral nervous system and in several extra nervous system neoplasms such as breast cancer, renal cancer, and melanoma. This antibody specially recognizes FABP7 and not cross-react with other FABPs.

Publications

Species	Application	Title
	IF	Nucleic Acids Res; H2A.Z.1 crosstalk with H3K56-acetylation controls gliogenesis through the transcription of folate receptor.; Authors - Libo Su
human	IHC	Ther Adv Med Oncol; Development and external validation of a composite immune-clinical prognostic model associated with EGFR mutation in East-Asian patients with lung adenocarcinoma.; Authors - Chengming Liu