FABP7 Polyclonal antibody

FABP7 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 51010-1-AP

Synonyms

B FABP, BLBP, Brain lipid binding protein, FABP7, FABPB, Fatty acid binding protein 7, MRG

---

Validation Data Gallery

• 

  WB analysis using 51010-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51010-1-AP (FABP7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 51010-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 51010-1-AP (FABP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 51010-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 51010-1-AP (FABP7 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 51010-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 51010-1-AP (FABP7 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 51010-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 51010-1-AP (FABP7 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 51010-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 51010-1-AP (FABP7 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human malignant melanoma using 51010-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 51010-1-AP (FABP7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human malignant melanoma using 51010-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 51010-1-AP (FABP7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 51010-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 51010-1-AP (FABP7 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 51010-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 51010-1-AP (FABP7 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of rat brain using 51010-1-AP

  Immunofluorescent analysis of (-20°C Acetone) fixed rat brain tissue using FABP7 antibody (51010-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: fetal human brain tissue, human brain tissue, mouse adipose tissue
• Positive IHC detected in: human malignant melanoma tissue, human breast cancer tissue, human gliomas tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Product Information

51010-1-AP targets FABP7 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: fatty acid binding protein 7, brain
• Calculated Molecular Weight: 132aa,15 kDa; 166aa,19 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC012299
• Gene Symbol: FABP7
• Gene ID (NCBI): 2173
• RRID: AB_2100456
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FABP7 (brain fatty acid-binding protein; BLBP) is a member of the FABP family of lipid chaperones involved in the uptake and intracellular trafficking of fatty acids. FABP7 is expressed in radial glial cells during brain development. FABP7 is up-regulated in brain tumor tissue; its expression has been associated with decreased survival times and tumor progression in patients with astrocytoma, melanoma, basal-type breast cancer, and renal cell carcinoma. This antibody is useful to label radial gial cells.

Publications

Species	Application	Title
mouse	WB	ACS Nano; Nanointegrative In Situ Reprogramming of Tumor-Intrinsic Lipid Droplet Biogenesis for Low-Dose Radiation-Activated Ferroptosis Immunotherapy; Authors - Qiqi Zhang
mouse	WB	Biomaterials; MMP-12 siRNA improves the homeostasis of the small intestine and metabolic dysfunction in high-fat diet feeding-induced obese mice.; Authors - Mingming Song
human	IF	Nat Commun; Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors.; Authors - Reuven Edri
mouse	IHC	Cancer Res; Bcl-xL enforces a slow-cycling state necessary for survival in the nutrient-deprived microenvironment of pancreatic cancer.; Authors - Yogev Sela
rat	WB	Int J Biol Macromol; Soybean soluble polysaccharide prevents obesity in high-fat diet-induced rats via lipid metabolism regulation; Authors - Haiyan He
mouse	IHC	Front Immunol; RGC-32 Regulates Generation of Reactive Astrocytes in Experimental Autoimmune Encephalomyelitis.; Authors - Alexandru Tatomir