FABP2 Polyclonal antibody

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• KD/KO Validated

FABP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 21252-1-AP

Synonyms

Intestinal-type fatty acid-binding protein, I-FABP, Fatty acid-binding protein, intestinal, Fatty acid-binding protein 2, Fatty acid binding protein 2

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Validation Data Gallery

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  WB analysis using 21252-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21252-1-AP (FABP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 21252-1-AP

  WB result of FABP2 antibody (21252-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FABP2 transfected COLO 320 cells.

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  IHC staining of human colon cancer using 21252-1-AP

  Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 21252-1-AP (FABP2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 21252-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21252-1-AP (FABP2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 21252-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21252-1-AP (FABP2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse small intestine tissue, COLO 320 cells, rat small intestine
• Positive IHC detected in: human stomach cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 14 publications below

Product Information

21252-1-AP targets FABP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat, pig, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FABP2 fusion protein Ag15793
• Full Name: fatty acid binding protein 2, intestinal
• Calculated Molecular Weight: 132 aa, 15 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC069617
• Gene Symbol: FABP2
• Gene ID (NCBI): 2169
• RRID: AB_10734425
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FABP2, also known as Intestinal Fatty Acid Binding Protein (I-FABP), is a member of the Fatty Acid Binding Proteins (FABPs) family. The cytoplasmic content of FABP2 could be delivered into the systematic circulation once the death of enterocyte happens, thus the elevated FABP2 concentration in the blood has been shown in many human intestinal diseases. FABP2 is a water soluble cytosolic protein with a small molecular weight of 14-15 kDa, and it is initially located in the mature enterocytes of the small intestine (PMID: 26547205).

Publications

Species	Application	Title
mouse	WB	Cell Metab; AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.; Authors - Hui Luo
mouse	WB	Biomaterials; MMP-12 siRNA improves the homeostasis of the small intestine and metabolic dysfunction in high-fat diet feeding-induced obese mice.; Authors - Mingming Song
mouse	WB	Cell Mol Gastroenterol Hepatol; FACI Is a Novel CREB-H-Induced Protein That Inhibits Intestinal Lipid Absorption and Reverses Diet-Induced Obesity.; Authors - Yun Cheng
rat	WB	Int J Mol Sci; INT-767-A Dual Farnesoid-X Receptor (FXR) and Takeda G Protein-Coupled Receptor-5 (TGR5) Agonist Improves Survival in Rats and Attenuates Intestinal Ischemia Reperfusion Injury; Authors - Emilio Canovai
mouse	WB	Microb Biotechnol; Faecal microbiota transplantation-mediated jejunal microbiota changes halt high-fat diet-induced obesity in mice via retarding intestinal fat absorption; Authors - Luoyi Zhu
mouse	WB	Ecotoxicol Environ Saf; Heat stress affects fetal brain and intestinal function associated with the alterations of placental barrier in late pregnant mouse.; Authors - Huiduo Guo