F11R Polyclonal antibody

F11R Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 16183-1-AP

Synonyms

CD321, F11 receptor, F11R, JAM, JAM 1, JAM A, JAM1, JAMA, JAMA1, JCAM, Junctional adhesion molecule 1, Junctional adhesion molecule A, KAT, PAM 1, Platelet adhesion molecule 1, Platelet F11 receptor

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Validation Data Gallery

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  WB analysis of mouse liver using 16183-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16183-1-AP (F11R antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 16183-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16183-1-AP (F11R antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 16183-1-AP

  IP result of anti-F11R (IP:16183-1-AP, 4ug; Detection:16183-1-AP 1:300) with mouse liver tissue lysate 3000ug.

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  IHC staining of human breast cancer using 16183-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16183-1-AP (F11R antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 16183-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16183-1-AP (F11R antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse liver tissue, human peripheral blood platelets
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below

Product Information

16183-1-AP targets F11R in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: F11 receptor
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC001533
• Gene Symbol: F11R
• Gene ID (NCBI): 50848
• RRID: AB_2878226
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The F11 receptor (F11R) was first identified in human platelets as a 32/35 kDa protein duplex that serves as the receptor for a functional antibody that activates platelets. It seems to play a role in epithelial tight junction formation. It was also reported to function as a receptor for reovirus and a ligand for the integrin LFA1.

Publications

Species	Application	Title
human	WB,IHC	Front Oncol; The Long Non-Coding RNA HOXC-AS3 Promotes Glioma Progression by Sponging miR-216 to Regulate F11R Expression.; Authors - Yongshuai Li
human	WB	Clin Exp Pharmacol Physiol; Rabeprazole destroyed gastric epithelial barrier function through FOXF1/STAT3-mediated ZO-1 expression; Authors - Fangying Yang
rat	WB	J Chem Neuroanat; Matrine treatment induced an A2 astrocyte phenotype and protected the blood-brain barrier in CNS autoimmunity.; Authors - Yilei Jing
human	WB	Biomedicines; Identification and Validation of JAM-A as a Novel Prognostic and Immune Factor in Human Tumors; Authors - Tianyi Ren
mouse	WB	Int J Biol Macromol; Epicatechin and β-glucan from whole highland barley grain ameliorates hyperlipidemia associated with attenuating intestinal barrier dysfunction and modulating gut microbiota in high-fat-diet-fed mice; Authors - Zehua Liu