ERP29 Polyclonal antibody

ERP29 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 24344-1-AP

Synonyms

PDIA9, PDI DB, ERp31, ERp28, ERP 29

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Validation Data Gallery

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  WB analysis using 24344-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24344-1-AP (ERP29 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 24344-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24344-1-AP (ERP29 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 24344-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24344-1-AP (ERP29 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 24344-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24344-1-AP (ERP29 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 24344-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 24344-1-AP (ERP29 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 24344-1-AP

  IP result of anti-ERP29 (IP:24344-1-AP, 4ug; Detection:24344-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of human liver cancer using 24344-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24344-1-AP (ERP29 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 24344-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24344-1-AP (ERP29 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 24344-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24344-1-AP (ERP29 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 24344-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24344-1-AP (ERP29 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 24344-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 24344-1-AP (ERP29 Antibody) at dilution of 1:50.

• 

  IHC staining of human pancreas using 24344-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 24344-1-AP (ERP29 Antibody) at dilution of 1:50.

• 

  IF Staining of human liver cancer using 24344-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using ERP29 antibody (24344-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human liver cancer using 24344-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using ERP29 antibody (24344-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 24344-1-AP

  Immunofluorescent analysis of HeLa cells using 24344-1-AP (ERP29 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: L02 cells, HEK-293 cells, mouse kidney tissue, mouse liver tissue, mouse lung tissue, rat testis tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human liver cancer tissue, human breast cancer tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human liver cancer tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

24344-1-AP targets ERP29 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ERP29 fusion protein Ag19451
• Full Name: endoplasmic reticulum protein 29
• Calculated Molecular Weight: 261 aa, 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC101495
• Gene Symbol: ERP29
• Gene ID (NCBI): 10961
• RRID: AB_2879503
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Endoplasmic reticulum resident protein 29 (ERP29) is a 29-kDa endoplasmic reticulum (ER) luminal protein. Structurally ERp29 is related to protein disulfide isomerases (PDI). However, it lacks the thioredoxin motif, suggesting that this protein does not function as a disulfide isomerase (PMID: 15500441). ERP29 plays an important role in the processing of secretory proteins within the (ER), possibly by participating in the folding of proteins in the ER. The secretion of thyroglobulin (TG) was found to be strongly regulated by ERP29 (PMID: 16380091).

Publications

Species	Application	Title
mouse	WB	iScience; Non-canonical function of DPP4 promotes cognitive impairment through ERp29-associated mitochondrial calcium overload in diabetes; Authors - Jiaxiu Li
mouse	WB,IF,CoIP	Metabolism; Nonenzymatic function of DPP4 promotes diabetes-associated cognitive dysfunction through IGF-2R/PKA/SP1/ERp29/IP3R2 pathway-mediated impairment of Treg function and M1 microglia polarization; Authors - Ya Hui

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