XPG Polyclonal antibody

• Featured Product
• KD/KO Validated

XPG Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 11331-1-AP

Synonyms

ERCC5, ERCM2, EC:3.1.-.-, DNA repair protein complementing XP-G cells, DNA excision repair protein ERCC-5

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Validation Data Gallery

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  WB analysis using 11331-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11331-1-AP (XPG antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11331-1-AP

  IP result of anti-XPG (IP:11331-1-AP, 4ug; Detection:11331-1-AP 1:500) with HeLa cells lysate 1200ug.

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  IF Staining of HeLa using 11331-1-AP

  Immunofluorescent analysis of HeLa cells using 11331-1-AP (XPG antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, Daudi cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IF: See 1 publications below

Product Information

11331-1-AP targets XPG in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: XPG fusion protein Ag1874
• Full Name: excision repair cross-complementing rodent repair deficiency, complementation group 5
• Calculated Molecular Weight: 1186 aa, 133 kDa
• Observed Molecular Weight: 200 kDa
• GenBank Accession Number: BC031522
• Gene Symbol: ERCC5
• Gene ID (NCBI): 2073
• RRID: AB_2098155
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The human genes correcting DNA repair defects are termed excision-repair cross-complementing or ERCC genes. The ERCC5 gene corrects the excision repair deficiency of Chinese hamster ovary cell line UV135 of complementation group 5. The human ERCC5 gene product is a structure-specific endonuclease required for making the 3-prime incision during DNA nucleotide excision-repair (NER). It also plays an important role in regulating DNA excision repair, removal of bulky lesions caused by environmental chemicals or UV light [PMID:22815677]. The calculated molecular weight of ERCC5 is 133 kDa, but the modified ERCC5 protein is about 200 kDa.

Publications

Species	Application	Title
human	WB	Cell; Human Rad52 Promotes XPG-Mediated R-loop Processing to Initiate Transcription-Associated Homologous Recombination Repair.; Authors - Takaaki Yasuhara ; KD Validated
human		Cancer Cell; Systematic Functional Annotation of Somatic Mutations in Cancer.; Authors - Patrick Kwok-Shing Ng
		Mol Carcinog; Associations between Expression Levels of Nucleotide Excision Repair Proteins in Lymphoblastoid Cells and Risk of Squamous Cell Carcinoma of the Head and Neck.; Authors - Peng Han
human	WB	Oncol Rep; Characterization of the nucleotide excision repair pathway and evaluation of compounds for overcoming the cisplatin resistance of non‑small cell lung cancer cell lines.; Authors - Toshihiro Suzuki ; KD Validated
human	WB	J Cancer; PMID: 31772670; Authors - Li-Ming Tan
human	WB	Biochim Biophys Acta; Stimulation of ribosomal RNA gene promoter by transcription factor Sp1 involves active DNA demethylation by Gadd45-NER pathway.; Authors - Pallavi Rajput