CoraLite® Plus 488-conjugated EPCAM/CD326 Polyclonal antibody

• Featured Product
• KD/KO Validated

EPCAM/CD326 Polyclonal Antibody for IF/ICC, FC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-21050

Synonyms

EPCAM, EGP, CO17 1A, CO 17A, Cell surface glycoprotein Trop-1

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Validation Data Gallery

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  IF Staining of MCF-7 using CL488-21050

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 EPCAM/CD326 antibody (CL488-21050) at dilution of 1:200.

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  FC experiment of MCF-7 using CL488-21050

  1x10^6 MCF-7 cells were surface stained with 0.8 ug CoraLite® Plus 488-conjugated EPCAM/CD326 Polyclonal antibody (CL488-21050) (red), or 0.8 ug Isotype Control (blue). Cells were not fixed.

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  FC experiment of A431 using CL488-21050

  1x10^6 A431 cells were surface stained with 0.4 ug CoraLite® Plus 488-conjugated EPCAM/CD326 Polyclonal antibody (CL488-21050) (red), or 0.4 ug Control Antibody (blue). Cells were not fixed.

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  FC experiment of HT-29 using CL488-21050

  1x10^6 HT-29 cells were surface stained with 0.8 ug CoraLite® Plus 488-conjugated EPCAM/CD326 Polyclonal antibody (CL488-21050) (red), or 0.8 ug Control Antibody (blue). Cells were not fixed.

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Tested Applications

• Positive IF/ICC detected in: MCF-7 cells
• Positive FC detected in: MCF-7 cells, A431 cells, HT-29 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-21050 targets EPCAM/CD326 in IF/ICC, FC applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EPCAM/CD326 fusion protein Ag15393
• Full Name: epithelial cell adhesion molecule
• Calculated Molecular Weight: 314 aa, 35 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC014785
• Gene Symbol: EPCAM
• Gene ID (NCBI): 4072
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)