NSE Polyclonal antibody

• Featured Product
• KD/KO Validated

NSE Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

32

Cat no : 10149-1-AP

Synonyms

ENO2, Neural enolase, Gamma enolase, Enolase 2, EC:4.2.1.11

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Validation Data Gallery

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  WB analysis using 10149-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10149-1-AP (NSE antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-251 using 10149-1-AP

  WB result of NSE antibody (10149-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSE transfected U-251 cells.

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  WB analysis of human brain using 10149-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10149-1-AP (NSE antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10149-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10149-1-AP (NSE antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10149-1-AP

  IP result of anti-NSE (IP:10149-1-AP, 4ug; Detection:10149-1-AP 1:300) with mouse brain tissue lysate 4000ug.

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  IHC staining of human brain using 10149-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10149-1-AP (NSE antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 10149-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10149-1-AP (NSE antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 10149-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10149-1-AP (NSE antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 10149-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10149-1-AP (NSE antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 10149-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 10149-1-AP (NSE antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 10149-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 10149-1-AP (NSE antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 10149-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NSE antibody (10149-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue, human brain tissue, U-251 cells, SH-SY5Y cells, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human brain tissue, human lung tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 18 publications below
• IHC: See 7 publications below
• IF: See 13 publications below

Product Information

10149-1-AP targets NSE in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, goat, duck
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NSE fusion protein Ag0196
• Full Name: enolase 2 (gamma, neuronal)
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC002745
• Gene Symbol: NSE
• Gene ID (NCBI): 2026
• RRID: AB_2099180
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NSE, also named as ENO2, belongs to the enolase family. Enolases are cytoplasmic glycolytic enzymes that may be involved in differentiation. The enolase has three isoenzymes, alpha, beta and gamma. The alpha form is expressed in most tissues, whereas the beta form is expressed in muscle tissue. The gamma enolase (ENO2), a homodimer, is primarily localized in neurons and neuroendocrine cells and is a cancer diagnostic marker for brain tumors (PMID:7520111). ENO2 plays a role in the glycolysis-related energy pathway and might be involved in higher metabolic activity during the day than at night, at least in part.

Publications

Species	Application	Title
mouse	IF	Nat Neurosci; Aerobic glycolysis is the predominant means of glucose metabolism in neuronal somata, which protects against oxidative damage; Authors - Yao Wei
mouse	WB	Mater Today Bio; In situ forming and biocompatible hyaluronic acid hydrogel with reactive oxygen species-scavenging activity to improve traumatic brain injury repair by suppressing oxidative stress and neuroinflammation.; Authors - Dan Zhang
mouse	WB	Mater Today Bio; Injectable hyaluronic acid hydrogel loaded with BMSC and NGF for traumatic brain injury treatment.; Authors - Luyu Wang
human	WB	Aging (Albany NY); Adipose-derived stromal cells improve functional recovery after spinal cord injury through TGF-β1/Smad3/PLOD2 pathway activation.; Authors - Fang Li
goat	IF	Cell Cycle; Effects of the histone acetylase inhibitor C646 on growth and differentiation of adipose-derived stem cells.; Authors - Zhimin Wang
human	WB	Cell Biosci; Profile of chimeric RNAs and TMPRSS2-ERG e2e4 isoform in neuroendocrine prostate cancer; Authors - Qiong Wang