ENO1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ENO1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

70

Reviews (2)

Cat no : 11204-1-AP

Synonyms

Enolase 1, ENO1L1, EC:4.2.1.11, C-myc promoter-binding protein, Alpha-enolase

---

Validation Data Gallery

• 

  WB analysis using 11204-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11204-1-AP (ENO1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11204-1-AP

  WB result of ENO1 antibody (11204-1-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ENO1 transfected HeLa cells.

• 

  WB analysis of human skeletal muscle using 11204-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11204-1-AP (ENO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 11204-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11204-1-AP (ENO1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 11204-1-AP

  IP result of anti-ENO1 (IP:11204-1-AP, 4ug; Detection:11204-1-AP 1:8000) with mouse skeletal muscle tissue lysate 1280 ug.

• 

  IHC staining of human stomach cancer using 11204-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11204-1-AP (ENO1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 11204-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11204-1-AP (ENO1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11204-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11204-1-AP (ENO1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11204-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11204-1-AP (ENO1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11204-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 11204-1-AP (ENO1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 11204-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 11204-1-AP (ENO1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 11204-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using ENO1 antibody (11204-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of A549 using 11204-1-AP

  1X10^6 A549 cells were intracellularly stained with 0.2 ug Anti-Human ENO1 (11204-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive WB detected in: HeLa cells, human skeletal muscle tissue, mouse skeletal muscle tissue, L02 cells, PC-3 cells, SGC-7901 cells, mouse liver tissue
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human stomach cancer tissue, human colon cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 11 publications below
• WB: See 59 publications below
• IHC: See 16 publications below
• IF: See 6 publications below
• IP: See 4 publications below
• CoIP: See 1 publications below

Product Information

11204-1-AP targets ENO1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ENO1 fusion protein Ag1692
• Full Name: enolase 1, (alpha)
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC015641
• Gene Symbol: ENO1
• Gene ID (NCBI): 2023
• RRID: AB_2099064
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Enolase is an important glycolytic enzyme involved in the interconversion of 2-phosphoglycerate to phosphoenolpyruvate. Enolase were down-regulated in oxLDL-treated cells or in VLDL-treated cells. And it identified which are associated with glucose metabolism were down-regulated in the process of foam cells formation. (PMID: 19756395)

Publications

Species	Application	Title
human	WB	Cancer Cell; Integrative plasma and fecal metabolomics identify functional metabolites in adenoma-colorectal cancer progression and as early diagnostic biomarkers; Authors - Yang Sun ; KD Validated
mouse	WB	Nat Neurosci; Metabolic lactate production coordinates vasculature development and progenitor behavior in the developing mouse neocortex.; Authors - Xiaoxiang Dong
human	WB,IF,IP	Mol Cell; The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.; Authors - Youming Zhu
mouse	WB,IP	Mol Cell; Riboregulation of Enolase 1 activity controls glycolysis and embryonic stem cell differentiation.; Authors - Ina Huppertz
human	WB	Nat Commun; Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.; Authors - Xiao-Hui Wang
human	WB	Nucleic Acids Res; FAX-RIC enables robust profiling of dynamic RNP complex formation in multicellular organisms in vivo.; Authors - Yongwoo Na

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Kihsu (Verified Customer) (06-10-2021)

its gave good looking results in WB

• Applications: Western Blot
• Cell Tissue Type: mouse liver tissues

FH

Kristian (Verified Customer) (06-07-2021)

Enolase 1 (green) is detected in the proximal tubules where HIF1A-signaling is activated.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Transgenic mouse kidney (TRACK)

ENO1 Antibody Immunofluorescence validation (1:200 dilution) in Transgenic mouse kidney (TRACK) (Cat no:11204-1-AP)

Related Products

PGK1 Polyclonal antibody

17811-1-AP

GAPDH Monoclonal antibody

60004-1-Ig

PKM Polyclonal antibody

25659-1-AP

TPI1 Polyclonal antibody

10713-1-AP

c-MYC Polyclonal antibody

10828-1-AP