Tested Applications
| Positive WB detected in | HeLa cells, human skeletal muscle tissue, mouse skeletal muscle tissue, L02 cells, PC-3 cells, SGC-7901 cells, mouse liver tissue |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human stomach cancer tissue, human colon cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11204-1-AP targets ENO1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1692 Product name: Recombinant human ENO1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2-434 aa of BC015641 Sequence: SILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELRDNDKTRYMGKGVSKAVEHINKTIAPALVSKKLNVTEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGKYDLDFKSPDDPSRYISPDQLADLYKSFIKDYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNEKSCNCLLLKVNQIGSVTESLQACKLAQANGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLLRIEEELGSKAKFAGRNFRNPLAK Predict reactive species |
| Full Name | enolase 1, (alpha) |
| Calculated Molecular Weight | 47 kDa |
| Observed Molecular Weight | 47 kDa |
| GenBank Accession Number | BC015641 |
| Gene Symbol | ENO1 |
| Gene ID (NCBI) | 2023 |
| ENSEMBL Gene ID | ENSG00000074800 |
| RRID | AB_2099064 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P06733 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Enolase is an important glycolytic enzyme involved in the interconversion of 2-phosphoglycerate to phosphoenolpyruvate. Enolase were down-regulated in oxLDL-treated cells or in VLDL-treated cells. And it identified which are associated with glucose metabolism were down-regulated in the process of foam cells formation. (PMID: 19756395)
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for ENO1 antibody 11204-1-AP | Download protocol |
| IF protocol for ENO1 antibody 11204-1-AP | Download protocol |
| IHC protocol for ENO1 antibody 11204-1-AP | Download protocol |
| IP protocol for ENO1 antibody 11204-1-AP | Download protocol |
| WB protocol for ENO1 antibody 11204-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Rabbit Monoclonal Antibody to ENO1 (Cat# 11204-1-AP) has 126 total citations published in journals including Nature Communications, Nature Cancer, Nature Metabolism, Cell Metabolism, Cell Research, Molecular Cell, EMBO Journal, Cancer Research, Cell Death & Disease, and Advanced Science. Associated research fields include cancer glycolysis/Warburg effect, tumor progression, ferroptosis, RNA–protein interactions, metabolic reprogramming, neurobiology, cardiology, immunology, and proteomics.
| Species | Application | Title |
|---|---|---|
Cancer Cell Integrative plasma and fecal metabolomics identify functional metabolites in adenoma-colorectal cancer progression and as early diagnostic biomarkers
| ||
J Hematol Oncol Alpha-enolase promotes cell glycolysis, growth, migration, and invasion in non-small cell lung cancer through FAK-mediated PI3K/AKT pathway.
| ||
Mol Cancer Alpha-enolase as a potential cancer prognostic marker promotes cell growth, migration, and invasion in glioma. | ||
Cell Metab The diapause-like colorectal cancer cells induced by SMC4 attenuation are characterized by low proliferation and chemotherapy insensitivity | ||
Cell Res cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions. | ||
Nat Cancer ENO1 suppresses cancer cell ferroptosis by degrading the mRNA of iron regulatory protein 1.
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Reviews
What customers say
All three reviewers report positive results. Two used the antibody for Western blot—one at 1:1000 on cancer cells ("works great," 5/5) and another on mouse liver tissue ("good looking results," 4/5). The third performed immunofluorescence at 1:200 on transgenic mouse kidney, successfully detecting Enolase 1 in proximal tubules associated with HIF1A signaling activation (5/5). Overall, users are satisfied with performance across both WB and IF applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sonam (Verified Customer) (01-27-2026) | Works great
|
Kihsu (Verified Customer) (06-10-2021) | its gave good looking results in WB
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Kristian (Verified Customer) (06-07-2021) | Enolase 1 (green) is detected in the proximal tubules where HIF1A-signaling is activated.
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