Emerin Recombinant antibody, PBS Only

Emerin Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230089B1

Cat no : 82888-1-PBS

Synonyms

EMD, STA, LEMD5, EDMD, 230089B1

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Validation Data Gallery

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  IF Staining of HeLa using 82888-1-RR (same clone as 82888-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Emerin antibody (82888-1-RR, Clone: 230089B1 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).(CL488-Phalloidin, green). This data was developed using the same antibody clone with 82888-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 82888-1-RR (same clone as 82888-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Emerin Recombinant antibody (82888-1-RR, Clone:230089B1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82888-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82888-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82888-1-RR against Human Emerin were performed. The affinity constant is 5.36 nM.

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Product Information

82888-1-PBS targets Emerin in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Emerin fusion protein Ag0236
• Full Name: emerin
• Calculated Molecular Weight: 34 kDa
• GenBank Accession Number: BC000738
• Gene Symbol: EMD
• Gene ID (NCBI): 2010
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Emerin (Emery-Dreifuss muscular dystrophy) (EMD or EDMD), a serine-rich nuclear membrane protein, is a member of the nuclear lamina-associated protein family. EMD may mediate membrane anchorage to the cytoskeleton by stabilizing and promoting the formation of a nuclear actin cortical network. Defects in EMD gene are the cause of Emery-Dreifuss muscular dystrophy type 1 (EDMD1), a degenerative myopathy characterized by weakness and atrophy of muscle without involvement of the nervous system, early contractures of the elbows Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects. EMD inhibits beta-catenin activity by preventing its accumulation in the nucleus and is involved in HIV-1 infection.