EIF2C1-Specific Polyclonal antibody, PBS Only

EIF2C1-Specific Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 19690-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HeLa using 19690-1-AP (same clone as 19690-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19690-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 19690-1-AP (same clone as 19690-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19690-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 19690-1-AP (same clone as 19690-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19690-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 19690-1-AP (same clone as 19690-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19690-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 19690-1-AP (same clone as 19690-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19690-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 19690-1-AP (same clone as 19690-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19690-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 19690-1-AP (same clone as 19690-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19690-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 19690-1-AP (same clone as 19690-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 19690-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 19690-1-AP (same clone as 19690-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 19690-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 19690-1-AP (same clone as 19690-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19690-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 19690-1-AP (same clone as 19690-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19690-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 19690-1-AP (same clone as 19690-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 19690-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 19690-1-AP (same clone as 19690-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 19690-1-PBS in a different storage buffer formulation.

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Product Information

19690-1-PBS targets EIF2C1-Specific in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: eukaryotic translation initiation factor 2C, 1
• Calculated Molecular Weight: 97 kDa
• Observed Molecular Weight: 97 kDa
• GenBank Accession Number: NM_012199
• Gene Symbol: EIF2C1
• Gene ID (NCBI): 26523
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

EIF2C1, also named as AGO1, belongs to the argonaute family and Ago subfamily. EIF2C1 is required for RNA-mediated gene silencing (RNAi). It binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. EIF2C1 may also be required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs). The antibody is specific to EIF2C1.