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EHMT2 Monoclonal antibody

EHMT2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A12E3

Cat no : 66689-1-Ig

Synonyms

EHMT2/G9a, Euchromatic histone-lysine N-methyltransferase 2, EC:2.1.1.-, C6orf30, BAT8



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:1500-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 8 publications below

Product Information

66689-1-Ig targets EHMT2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen EHMT2 fusion protein Ag27477
Full Name euchromatic histone-lysine N-methyltransferase 2
Calculated Molecular Weight 1210 aa, 132 kDa
Observed Molecular Weight 150 kDa and 170 kDa
GenBank Accession NumberBC018718
Gene Symbol EHMT2
Gene ID (NCBI) 10919
RRIDAB_2882043
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for EHMT2 antibody 66689-1-IgDownload protocol
IF protocol for EHMT2 antibody 66689-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Differ

Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration

Authors - Huaizhen Liang
humanWB

Nat Commun

PCGF6 controls neuroectoderm specification of human pluripotent stem cells by activating SOX2 expression.

Authors - Xianchun Lan
humanWB

Am J Cancer Res

HSF2 regulates aerobic glycolysis by suppression of FBP1 in hepatocellular carcinoma.

Authors - Li-Na Yang
humanWB

iScience

Linkage of methionine addiction, histone lysine hypermethylation, and malignancy.

Authors - Jun Yamamoto
humanWB

bioRxiv

Distinct functions of EHMT1 and EHMT2 in cancer chemotherapy and immunotherapy

Authors - Zhihua Kang
mouseWB

Front Cell Dev Biol

Emerin interacts with histone methyltransferases to regulate repressive chromatin at the nuclear periphery

Authors - Nicholas Marano

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Hongtao (Verified Customer) (12-17-2021)

This antibody works very well for human cancer cell whole cell lysate with 10 ug total protein input.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: human kidney cancer cell
EHMT2 Antibody Western Blot validation (1:1000 dilution) in human kidney cancer cell (Cat no:66689-1-Ig)