EHMT2 Monoclonal antibody

EHMT2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

6

CloneNo.

1A12E3

Reviews (1)

Cat no : 66689-1-Ig

Synonyms

BAT8, C6orf30, DKFZp686H08213, EHMT2, FLJ35547, G9A, H3 K9 HMTase 3, HLA B associated transcript 8, KMT1C, Lysine N methyltransferase 1C, NG36, NG36/G9a, Protein G9a

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Validation Data Gallery

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  WB analysis of HeLa using 66689-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66689-1-Ig (EHMT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66689-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66689-1-Ig (EHMT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66689-1-Ig

  WB result of EHMT2 antibody (66689-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EHMT2 transfected HEK-293 cells.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 6 publications below

Product Information

66689-1-Ig targets EHMT2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: EHMT2 fusion protein Ag27477
• Full Name: euchromatic histone-lysine N-methyltransferase 2
• Calculated molecular weight: 1210 aa, 132 kDa
• Observed molecular weight: 150 kDa and 170 kDa
• GenBank accession number: BC018718
• Gene symbol: EHMT2
• Gene ID (NCBI): 10919
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; PCGF6 controls neuroectoderm specification of human pluripotent stem cells by activating SOX2 expression.; Authors - Xianchun Lan
human	WB	Am J Cancer Res; HSF2 regulates aerobic glycolysis by suppression of FBP1 in hepatocellular carcinoma.; Authors - Li-Na Yang
human	WB	iScience; Linkage of methionine addiction, histone lysine hypermethylation, and malignancy.; Authors - Jun Yamamoto
mouse	WB	Front Cell Dev Biol; Emerin interacts with histone methyltransferases to regulate repressive chromatin at the nuclear periphery; Authors - Nicholas Marano
human	WB	Cell J; High Expression of G9a Induces Cisplatin Resistance in Hepatocellular Carcinoma; Authors - Junhao Fu
human	WB	Cell Insight; The CHCHD2/Sirt1 corepressors involve in G9a-mediated regulation of RNase H1 expression to control R-loop; Authors - Le Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hongtao (Verified Customer) (12-17-2021)

This antibody works very well for human cancer cell whole cell lysate with 10 ug total protein input.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human kidney cancer cell

EHMT2 Antibody Western Blot validation (1:1000 dilution) in human kidney cancer cell (Cat no:66689-1-Ig)