EHMT2 Monoclonal antibody

EHMT2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A12E3

Cat no : 66689-1-Ig

Synonyms

BAT8, C6orf30, DKFZp686H08213, EHMT2, FLJ35547, G9A, H3 K9 HMTase 3, HLA B associated transcript 8, KMT1C, Lysine N methyltransferase 1C, NG36, NG36/G9a, Protein G9a



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 6 publications below

Product Information

66689-1-Ig targets EHMT2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen EHMT2 fusion protein Ag27477
Full Name euchromatic histone-lysine N-methyltransferase 2
Calculated molecular weight 1210 aa, 132 kDa
Observed molecular weight 150 kDa and 170 kDa
GenBank accession numberBC018718
Gene symbol EHMT2
Gene ID (NCBI) 10919
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for EHMT2 antibody 66689-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

PCGF6 controls neuroectoderm specification of human pluripotent stem cells by activating SOX2 expression.

Authors - Xianchun Lan
humanWB

Am J Cancer Res

HSF2 regulates aerobic glycolysis by suppression of FBP1 in hepatocellular carcinoma.

Authors - Li-Na Yang
humanWB

iScience

Linkage of methionine addiction, histone lysine hypermethylation, and malignancy.

Authors - Jun Yamamoto
mouseWB

Front Cell Dev Biol

Emerin interacts with histone methyltransferases to regulate repressive chromatin at the nuclear periphery

Authors - Nicholas Marano
humanWB

Cell J

High Expression of G9a Induces Cisplatin Resistance in Hepatocellular Carcinoma

Authors - Junhao Fu
humanWB

Cell Insight

The CHCHD2/Sirt1 corepressors involve in G9a-mediated regulation of RNase H1 expression to control R-loop

Authors - Le Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Hongtao (Verified Customer) (12-17-2021)

This antibody works very well for human cancer cell whole cell lysate with 10 ug total protein input.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: human kidney cancer cell
EHMT2 Antibody Western Blot validation (1:1000 dilution) in human kidney cancer cell (Cat no:66689-1-Ig)