EHMT2 Monoclonal antibody

• Featured Product
• KD/KO Validated

EHMT2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

8

CloneNo.

1A12E3

Reviews (1)

Cat no : 66689-1-Ig

Synonyms

EHMT2/G9a, Euchromatic histone-lysine N-methyltransferase 2, EC:2.1.1.-, C6orf30, BAT8

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Validation Data Gallery

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  WB analysis of HeLa using 66689-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66689-1-Ig (EHMT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66689-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66689-1-Ig (EHMT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66689-1-Ig

  WB result of EHMT2 antibody (66689-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EHMT2 transfected HEK-293 cells.

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  IF Staining of A431 using 66689-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using EHMT2 antibody (66689-1-Ig, Clone: 1A12E3 ) at dilution of 1:3000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1500-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below

Product Information

66689-1-Ig targets EHMT2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: EHMT2 fusion protein Ag27477
• Full Name: euchromatic histone-lysine N-methyltransferase 2
• Calculated Molecular Weight: 1210 aa, 132 kDa
• Observed Molecular Weight: 150 kDa and 170 kDa
• GenBank Accession Number: BC018718
• Gene Symbol: EHMT2
• Gene ID (NCBI): 10919
• RRID: AB_2882043
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell Death Differ; Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration; Authors - Huaizhen Liang
human	WB	Nat Commun; PCGF6 controls neuroectoderm specification of human pluripotent stem cells by activating SOX2 expression.; Authors - Xianchun Lan
human	WB	Am J Cancer Res; HSF2 regulates aerobic glycolysis by suppression of FBP1 in hepatocellular carcinoma.; Authors - Li-Na Yang
human	WB	iScience; Linkage of methionine addiction, histone lysine hypermethylation, and malignancy.; Authors - Jun Yamamoto
human	WB	bioRxiv; Distinct functions of EHMT1 and EHMT2 in cancer chemotherapy and immunotherapy; Authors - Zhihua Kang
mouse	WB	Front Cell Dev Biol; Emerin interacts with histone methyltransferases to regulate repressive chromatin at the nuclear periphery; Authors - Nicholas Marano

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hongtao (Verified Customer) (12-17-2021)

This antibody works very well for human cancer cell whole cell lysate with 10 ug total protein input.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human kidney cancer cell

EHMT2 Antibody Western Blot validation (1:1000 dilution) in human kidney cancer cell (Cat no:66689-1-Ig)