Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:1500-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66689-1-Ig targets EHMT2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27477 Product name: Recombinant human EHMT2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 652-1001 aa of BC018718 Sequence: ESYHDCVLLFLSRGANPELRNKEGDTAWDLTPERSDVWFALQLNRKLRLGVGNRAIRTEKIICRDVARGYENVPIPCVNGVDGEPCPEDYKYISENCETSTMNIDRNITHLQHCTCVDDCSSSNCLCGQLSIRCWYDKDGRLLQEFNKIEPPLIFECNQACSCWRNCKNRVVQSGIKVRLQLYRTAKMGWGVRALQTIPQGTFICEYVGELISDAEADVREDDSYLFDLDNKDGEVYCIDARYYGNISRFINHLCDPNIIPVRVFMLHQDLRFPRIAFFSSRDIRTGEELGFDYGDRFWDIKSKYFTCQCGSEKCKHSAEAIALEQSRLARLDPHPELLPELGSLPPVNT Predict reactive species |
| Full Name | euchromatic histone-lysine N-methyltransferase 2 |
| Calculated Molecular Weight | 1210 aa, 132 kDa |
| Observed Molecular Weight | 150 kDa and 170 kDa |
| GenBank Accession Number | BC018718 |
| Gene Symbol | EHMT2 |
| Gene ID (NCBI) | 10919 |
| RRID | AB_2882043 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q96KQ7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
EHMT2 (Euchromatic Histone Lysine Methyltransferase 2), also known as G9a, is a key epigenetic regulatory enzyme. EHMT2 contains a SET domain, which is responsible for its methyltransferase activity. EHMT2 primarily functions by forming heterodimers with its homolog, EHMT1 (also known as GLP); together, these two proteins are responsible for the mono- and dimethylation of lysine 9 on histone H3 (H3K9me1 and H3K9me2).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for EHMT2 antibody 66689-1-Ig | Download protocol |
| WB protocol for EHMT2 antibody 66689-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The EHMT2 Monoclonal antibody (1A12E3), catalog number 66689-1-Ig, has 12 citations published in journals including Nature Communications, Cell Death & Differentiation, Cell Death & Disease, European Journal of Medicinal Chemistry, Cell Insight, Biomolecules, Frontiers in Cell and Developmental Biology, iScience, Computational Biology and Chemistry, American Journal of Cancer Research, Cell Journal, and bioRxiv. Research fields span epigenetics, histone methylation, cancer biology (breast cancer, hepatocellular carcinoma), stem cell specification, autophagy, and chemotherapy resistance.
| Species | Application | Title |
|---|---|---|
Nat Commun PCGF6 controls neuroectoderm specification of human pluripotent stem cells by activating SOX2 expression. | ||
Cell Death Differ Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration | ||
Cell Death Dis The EHMT2-MBLAC2 axis suppresses ribosomal DNA transcription in response to nucleolar DNA damage.
| ||
Eur J Med Chem Discovery of a potent PROTAC targeting G9a suppresses proliferation of triple-negative breast cancer cells and psoriasis-like keratinocytes. | ||
Cell Insight The CHCHD2/Sirt1 corepressors involve in G9a-mediated regulation of RNase H1 expression to control R-loop | ||
Biomolecules Targeting G9a Exerts Pleiotropic Suppression in Triple-Negative Breast Cancer Cells: Cooperatively Inducing Pyroptosis and Apoptosis.
|
Reviews
What customers say
Both reviewers used this antibody for Western Blot at a 1:1000 dilution and reported positive results. One user tested it on HEK cells and simply rated it "great" with a 5-star score. The other validated it on human kidney cancer cell lysates (10 µg total protein input) and confirmed it works very well, giving it 4 stars. Overall, users find this antibody reliable and effective for Western Blot applications on human cell lines.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
aditya (Verified Customer) (03-24-2026) | great
|
Hongtao (Verified Customer) (12-17-2021) | This antibody works very well for human cancer cell whole cell lysate with 10 ug total protein input.
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