PHD1 Polyclonal antibody

PHD1 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (1)





Cat no : 12984-1-AP


Egl nine homolog 2, EGLN2, EIT6, Estrogen induced tag 6, HIF PH1, HIF prolyl hydroxylase 1, HIFPH1, HPH 1, HPH 3, PHD1

Tested Applications

Positive WB detected inmouse testis tissue, HEK-293 cells, HeLa cells, human spleen tissue, mouse heart tissue, PC-3 cells
Positive IF detected inPC-3 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12984-1-AP targets PHD1 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHD1 fusion protein Ag3616
Full Name egl nine homolog 2 (C. elegans)
Calculated Molecular Weight 407 aa, 44 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC036051
Gene Symbol EGLN2
Gene ID (NCBI) 112398
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EGLN2, also named as PHD1, EIT6 and HPH-3, catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. EGLN2 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. It may play a role in cell growth regulation.


Product Specific Protocols
WB protocol for PHD1 antibody 12984-1-APDownload protocol
IF protocol for PHD1 antibody 12984-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Res

Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation

Authors - Yunzi Mao

Respir Res

Short isoform thymic stromal lymphopoietin reduces inflammation and aerobic glycolysis of asthmatic airway epithelium by antagonizing long isoform thymic stromal lymphopoietin.

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J Cell Mol Med

LncRNA MIAT enhances cerebral ischaemia/reperfusion injury in rat model via interacting with EGLN2 and reduces its ubiquitin-mediated degradation.

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Asian J Androl

CHD1 deletion stabilizes HIF1α to promote angiogenesis and glycolysis in prostate cancer

Authors - Yu-Zhao Wang

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Cancer associated fibroblast-derived CCL5 promotes hepatocellular carcinoma metastasis through activating HIF1α/ZEB1 axis.

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