ECHS1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ECHS1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

28

Cat no : 11305-1-AP

Synonyms

mECH1, mECH, Enoyl-CoA hydratase, mitochondrial, Enoyl-CoA hydratase 1, ECHM

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Validation Data Gallery

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  WB analysis of MCF-7 using 11305-1-AP

  WB result of ECHS1 antibody (11305-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ECHS1 transfected MCF-7 cells.

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  WB analysis using 11305-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11305-1-AP (ECHS1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11305-1-AP

  WB result of ECHS1 antibody (11305-1-AP, 1:1200) with si-Control and si-ECHS1 transfected HepG2 cells. .

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  WB analysis of HeLa using 11305-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11305-1-AP (ECHS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 11305-1-AP

  IP result of anti-ECHS1 (IP:11305-1-AP, 3ug; Detection:11305-1-AP 1:500) with PC-3 cells lysate 3000ug.

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  IHC staining of human colon using 11305-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11305-1-AP (ECHS1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 11305-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11305-1-AP (ECHS1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 11305-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11305-1-AP (ECHS1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 11305-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ECHS1 antibody (11305-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, MCF-7 cells, mouse heart, mouse liver, rat liver
• Positive IP detected in: PC-3 cells
• Positive IHC detected in: human colon tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:600-1:2400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 25 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

11305-1-AP targets ECHS1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ECHS1 fusion protein Ag1842
• Full Name: enoyl Coenzyme A hydratase, short chain, 1, mitochondrial
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 29-31 kDa
• GenBank Accession Number: BC008906
• Gene Symbol: ECHS1
• Gene ID (NCBI): 1892
• RRID: AB_2262166
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Enoyl-coenzyme A hydratase (ECHS1) is a mitochondrial protein which catalyzes the hydration of 2-trans-enoyl-coenzyme A intermediates to L-3-hydroxyacyl-coenzyme A, playing key role in metabolism of fatty acids in mitochondria. ECHS1 is highly expressed in muscle, liver and fibroblasts. Altered expression of ECHS1 has been considered as a characteristic feature of mitochondria dysfunction. (23275097, 23235493)

Publications

Species	Application	Title
human	WB	Cancer Res; One-hit effects in cancer: altered proteome of morphologically normal colon crypts in familial adenomatous polyposis.; Authors - Yeung Anthony T AT
rat	WB	J Proteome Res; Proteomic analysis of left ventricular remodeling in an experimental model of heart failure.; Authors - Cieniewski-Bernard Caroline C
human	WB	J Proteome Res; Novel Proteomic Biomarker Panel for Prediction of Aggressive Metastatic Hepatocellular Carcinoma Relapse in Surgically Resectable Patients.; Authors - Gek San Tan
human	WB	Oncotarget; Quantitative proteomics reveals molecular mechanism of gamabufotalin and its potential inhibition on Hsp90 in lung cancer.; Authors - Liyuan Zhang
mouse	WB	Am J Pathol; The Krebs cycle and mitochondrial mass are early victims of endothelial dysfunction: proteomic approach.; Authors - Addabbo Francesco F
human	WB	J Med Genet; Strategic validation of variants of uncertain significance in ECHS1 genetic testing; Authors - Yoshihito Kishita ; KO Validated