ECD Polyclonal antibody

• Featured Product
• KD/KO Validated

ECD Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 10192-1-AP

Synonyms

Protein ecdysoneless homolog, Human suppressor of GCR two, hSGT1

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Validation Data Gallery

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  WB analysis of HeLa using 10192-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10192-1-AP (ECD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10192-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10192-1-AP (ECD antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10192-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10192-1-AP (ECD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10192-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10192-1-AP (ECD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10192-1-AP

  IP result of anti-ECD (IP:10192-1-AP, 4ug; Detection:10192-1-AP 1:300) with HeLa cells lysate 2480ug.

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  IHC staining of human ovary cancer using 10192-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 10192-1-AP (ECD antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 10192-1-AP

  Immunofluorescent analysis of HeLa cells using 10192-1-AP (ECD antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

10192-1-AP targets ECD in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ECD fusion protein Ag0245
• Full Name: ecdysoneless homolog (Drosophila)
• Calculated Molecular Weight: 73 kDa
• Observed Molecular Weight: 73 kDa
• GenBank Accession Number: BC000721
• Gene Symbol: ECD
• Gene ID (NCBI): 11319
• RRID: AB_2188183
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The human suppressor of GCR two (hSGT1) was isolated by its ability to complement the growth defect of the gcr2 mutant of Saccharomyces cerevisiae. Further work confirmed that the rescue of growth was associated with recovery of glycolytic enzyme activities of gcr2, and that hSGT1 did not complement the growth defect of a gcr1 mutant. A hybrid protein comprising hSgt1p and the DNA-binding domain of Gal4p (GBD) activated a GAL1-lacZ reporter gene fusion, suggesting that the cloned gene may be a transcriptional activator. Two-hybrid experiments in yeast also indicate that hSgt1p interacts with Gcr1p. Northern analysis showed that hSGT1 is highly expressed in muscle and heart.

Publications

Species	Application	Title
human	WB	Oncotarget; Amplification of ACK1 promotes gastric tumorigenesis via ECDdependent p53 ubiquitination degradation.; Authors - Song-Hui Xu ; KD Validated