Tested Applications
| Positive WB detected in | U2OS cells, Raji cells, 3T3-L1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14159-1-AP targets EBF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5341 Product name: Recombinant human EBF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 4-350 aa of BC038805 Sequence: IQESIQRSGSSMKEEPLGSGMNAVRTWMQGAGVLDANTAAQSGVGLARAHFEKQPPSNLRKSNFFHFVLALYDRQGQPVEIERTAFVGFVEKEKEANSEKTNNGIHYRLQLLYSNGIRTEQDFYVRLIDSMTKQAIVYEGQDKNPEMCRVLLTHEIMCSRCCDKKSCGNRNETPSDPVIIDRFFLKFFLKCNQNCLKNAGNPRDMRRFQVVVSTTVNVDGHVLAVSDNMFVHNNSKHGRRARRLDPSEGTPSYLEHATPCIKAISPSEGWTTGGATVIIIGDNFFDGLQVIFGTMLVWSELITPHAIRVQTPPRHIPGVVEVTLSYKSKQFCKGTPGRFIYTALNEP Predict reactive species |
| Full Name | early B-cell factor 1 |
| Calculated Molecular Weight | 591 aa, 64 kDa |
| Observed Molecular Weight | 57-67 kDa |
| GenBank Accession Number | BC038805 |
| Gene Symbol | EBF1 |
| Gene ID (NCBI) | 1879 |
| RRID | AB_2231057 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UH73 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
EBF1, also named as O/E-1, OE-1, OLF1, COE1 and EBF, belongs to the COE family. It is a transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. EBF1 is a key factor for the specification of B-cell lineage.
Publications
What published studies show
EBF1 Polyclonal antibody (Cat# 14159-1-AP) has 3 total citations. The journals are Nature, ACS Nano, and iScience. The associated research fields include astrocyte reactivity and transcriptional regulation in neurological disorders, tumor microenvironment immunology with antitumor immune response, and brown adipose tissue biology related to osteogenic differentiation.
| Species | Application | Title |
|---|---|---|
ACS Nano Arginine Supplementation Targeting Tumor-Killing Immune Cells Reconstructs the Tumor Microenvironment and Enhances the Antitumor Immune Response | ||
iScience Whitening of brown adipose tissue inhibits osteogenic differentiation via secretion of S100A8/A9 |
