EBF1 Polyclonal antibody

EBF1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 14159-1-AP

Synonyms

COE1, Early B cell factor, early B cell factor 1, EBF, EBF1, O/E 1, OE 1, OLF1, Transcription factor COE1

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Validation Data Gallery

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  WB analysis of U2OS using 14159-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 14159-1-AP (EBF1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 14159-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14159-1-AP (EBF1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  WB analysis of 3T3-L1 using 14159-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 14159-1-AP (EBF1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 14159-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14159-1-AP (EBF1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 14159-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14159-1-AP (EBF1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: U2OS cells, Raji cells, 3T3-L1 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

14159-1-AP targets EBF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EBF1 fusion protein Ag5341
• Full Name: early B-cell factor 1
• Calculated Molecular Weight: 591 aa, 64 kDa
• Observed Molecular Weight: 57-67 kDa
• GenBank Accession Number: BC038805
• Gene Symbol: EBF1
• Gene ID (NCBI): 1879
• RRID: AB_2231057
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EBF1, also named as O/E-1, OE-1, OLF1, COE1 and EBF, belongs to the COE family. It is a transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. EBF1 is a key factor for the specification of B-cell lineage.

Publications

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
mouse	WB	iScience; Whitening of brown adipose tissue inhibits osteogenic differentiation via secretion of S100A8/A9; Authors - Ting Wang
mouse	WB	ACS Nano; Arginine Supplementation Targeting Tumor-Killing Immune Cells Reconstructs the Tumor Microenvironment and Enhances the Antitumor Immune Response; Authors - Junlei Zhang