ChromoTek Dnmt1-Chromobody® plasmid (TagRFP)

DNA plasmid encoding for anti-Dnmt1 VHH (anti-Dnmt1 Nanobody) fused to TagRFP.

Specificity

Dmnt1

Applications

IF, Live cell imaging

Vector type

Mammalian expression vector

Reporter

TagRFP

Publications

1

Cat no : dcr

Synonyms

Chromobody, Dmnt1 Chromobody

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Validation Data Gallery

• 

  Series of 4 images of HeLa cells expressing the Dnmt1-Chromobody (over 5.5 hours): In G phase cells, the signal is homogeneously distributed through the nucleus and cytoplasm. Over time, during S phase granules appear in the nucleus as replication foci form, finally the granularity disappears, and the cells divide.

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Product Information

DNA plasmid encoding for anti-Dnmt1 VHH (anti-Dnmt1 Nanobody) fused to TagRFP.

• Description: The Dnmt1-Chromobody visualizes endogenous Dnmt1 in real-time during cell cycle progression.

  • Trace dynamic changes during the cell cycle in real time

  • Monitor the distribution of an endogenous cell cycle marker protein without overexpression artifacts or cytotoxic effects from overexpressed fluorescent protein fusion Dnmt1

• Applications: Live cell imaging
• Specificity: Dnmt1 (DNA (cytosine-5)-methyltransferase 1), tested in human cells
• Reporter: TagRFP
• Vector type: Mammalian expression vector
• Promoter: Constitutive CMV IE
• Codon usage: Mammalian
• Selection: Kan/Neo
• Sequence: With the Cell Cycle-Chromobody plasmid you receive the sequence information of the Alpaca antibody to PCNA fused to TagRFP, as well as the full vector sequence.
• Transfection: Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.
• Microscopy techniques: Wide-field epifluorescence microscopy; confocal microscopy; super-resolution microscopy e.g. STED and 3D-SIM; high-content microscopy and analysis
• Storage Condition: Shipped at ambient temperature. Store at -20 °C

Publications

Application	Title
	Sci Rep; Long non-coding RNA PARTICLE bridges histone and DNA methylation.; Authors - Valerie Bríd O'Leary