Validation Data Gallery
|Positive WB detected in||human brain tissue, human heart tissue, Sp2/0 cells|
|Positive IP detected in||mouse skeletal muscle tissue|
|Positive IHC detected in||human pancreas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:300-1:600|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11954-1-AP targets DYNLT1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||DYNLT1 fusion protein Ag2557|
|Full Name||dynein, light chain, Tctex-type 1|
|Calculated molecular weight||113 aa, 12 kDa|
|Observed molecular weight||12 kDa|
|GenBank accession number||BC029412|
|Gene ID (NCBI)||6993|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
DYNLT (or Tctex-1) was originally described as a light chain component of the dynein motor complex. Tctex-1 also has several dynein-independent functions, including roles in G protein signaling activation and neuronal growth. Tctex-1 is selectively enriched in proliferating neural progenitors of both embryonic and adult brains. Genetic knockdown of Tctex-1 in radial precursors promoted neurogenesis, indicating its implication in regulation of cortical neurogenesis. (Pubmed: 19448628)
|Product Specific Protocols|
|WB protocol for DYNLT1 antibody 11954-1-AP||Download protocol|
|IHC protocol for DYNLT1 antibody 11954-1-AP||Download protocol|
|IF protocol for DYNLT1 antibody 11954-1-AP||Download protocol|
|IP protocol for DYNLT1 antibody 11954-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Lfc and Tctex-1 regulate the genesis of neurons from cortical precursor cells.
Dlic1 deficiency impairs ciliogenesis of photoreceptors by destabilizing dynein.
J Cell Biol
Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway.
Dynein Light Chain Protein Tctex1: A Novel Prognostic Marker and Molecular Mediator in Glioblastoma.
Cannabinoid receptor-2 expression modulates Gβ1γ2 protein interaction with the activator of G protein signalling 2/dynein light chain protein Tctex-1.
J Cell Physiol
Tctex-1, a novel interaction partner of Kidney Injury Molecule-1, is required for efferocytosis.