DYNLT1 Polyclonal antibody

• Featured Product
• KD/KO Validated

DYNLT1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 11954-1-AP

Synonyms

CW 1, DYNLT1, Protein CW 1, TCTEL1, tctex 1, TCTEX1

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Validation Data Gallery

• 

  WB analysis of human brain using 11954-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11954-1-AP (DYNLT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 11954-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11954-1-AP (DYNLT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Sp2/0 using 11954-1-AP

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 11954-1-AP (DYNLT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 11954-1-AP

  IP Result of anti-DYNLT1 (IP:11954-1-AP, 4ug; Detection:11954-1-AP 1:300) with mouse skeletal muscle tissue lysate 3600ug.

• 

  IHC staining of human pancreas using 11954-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 11954-1-AP (DYNLT1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 11954-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 11954-1-AP (DYNLT1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 11954-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 11954-1-AP (DYNLT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human brain tissue, human heart tissue, Sp2/0 cells
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:300-1:600
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Product Information

11954-1-AP targets DYNLT1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DYNLT1 fusion protein Ag2557
• Full Name: dynein, light chain, Tctex-type 1
• Calculated molecular weight: 113 aa, 12 kDa
• Observed molecular weight: 12 kDa
• GenBank accession number: BC029412
• Gene symbol: DYNLT1
• Gene ID (NCBI): 6993
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DYNLT (or Tctex-1) was originally described as a light chain component of the dynein motor complex. Tctex-1 also has several dynein-independent functions, including roles in G protein signaling activation and neuronal growth. Tctex-1 is selectively enriched in proliferating neural progenitors of both embryonic and adult brains. Genetic knockdown of Tctex-1 in radial precursors promoted neurogenesis, indicating its implication in regulation of cortical neurogenesis. (Pubmed: 19448628)

Publications

Species	Application	Title
mouse	WB,IHC,IF	Nat Neurosci; Lfc and Tctex-1 regulate the genesis of neurons from cortical precursor cells.; Authors - Gauthier-Fisher Andrée A ; KD Validated
mouse	WB	Cell Res; Dlic1 deficiency impairs ciliogenesis of photoreceptors by destabilizing dynein.; Authors - Kong Shanshan S
human	WB	J Cell Biol; Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway.; Authors - Hironori Inaba ; KD Validated
human	IHC	Cancers (Basel); Dynein Light Chain Protein Tctex1: A Novel Prognostic Marker and Molecular Mediator in Glioblastoma.; Authors - Claudia Alexandra Dumitru
	WB	Biochem Pharmacol; Cannabinoid receptor-2 expression modulates Gβ1γ2 protein interaction with the activator of G protein signalling 2/dynein light chain protein Tctex-1.; Authors - Marina Nagler
human	WB,IF	J Cell Physiol; Tctex-1, a novel interaction partner of Kidney Injury Molecule-1, is required for efferocytosis.; Authors - Ola Z Ismail ; KD Validated

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