DYNC2LI1 Polyclonal antibody

DYNC2LI1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 15949-1-AP

Synonyms

CGI 60, D2LIC, DYNC2LI1, LIC3

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Validation Data Gallery

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  WB analysis using 15949-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15949-1-AP (DYNC2LI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293T cells, L02 cells, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 2 publications below

Product Information

15949-1-AP targets DYNC2LI1 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DYNC2LI1 fusion protein Ag8584
• Full Name: dynein, cytoplasmic 2, light intermediate chain 1
• Calculated Molecular Weight: 352 aa, 40 kDa
• Observed Molecular Weight: 37-40 kDa, 22 kDa
• GenBank Accession Number: BC006969
• Gene Symbol: DYNC2LI1
• Gene ID (NCBI): 51626
• RRID: AB_2093643
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DYNC2LI1 (D2LIC, LIC3) is a member of cytoplasmic dynein 2 complex that is required for retrograde transport. It is ubiquitously expressed with high expression levels in brain, kidney, lung and testes. In mammalian cells, DYNC2LI1 is localized to centrosomal region Mutations in DYNC2LI1 disrupt cilia function and cause various skeletal ciliopathies. There're some isoforms with MW 40 kDa, 38 kDa, 22 kDa and 16 kDa.

Publications

Species	Application	Title
human	IF	Elife; Dynein-2 intermediate chains play crucial but distinct roles in primary cilia formation and function.; Authors - Laura Vuolo
mouse	WB	Hum Mol Genet; Dynll1 is essential for development and promotes endochondral bone formation by regulating intraflagellar Dynein function in primary cilia.; Authors - Ashleigh King
human	WB	FEBS Lett; Recombinant human cytoplasmic dynein heavy chain 1 and 2: observation of dynein-2 motor activity in vitro.; Authors - Ichikawa Muneyoshi M
human	IF	Sci Rep; DYNC2LI1 mutations broaden the clinical spectrum of dynein-2 defects.; Authors - Kristin Kessler
human	WB	Cilia; Mutations in IFT-A satellite core component genes IFT43 and IFT121 produce short rib polydactyly syndrome with distinctive campomelia.; Authors - Ivan Duran
human	WB	J Cell Sci; Roles for CEP170 in cilia function and dynein-2 assembly; Authors - Johannes F Weijman