DYNC1LI1 Polyclonal antibody

DYNC1LI1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25326-1-AP

Synonyms

LIC1, DNCLI1, DLIC-1, DLC-A, DLCA

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Validation Data Gallery

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  WB analysis of K-562 using 25326-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25326-1-AP (DYNC1LI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 25326-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25326-1-AP (DYNC1LI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 25326-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25326-1-AP (DYNC1LI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse pancreas using 25326-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 25326-1-AP (DYNC1LI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 25326-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 25326-1-AP (DYNC1LI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of K-562 using 25326-1-AP

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using DYNC1LI1 antibody (25326-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: K-562 cells, HeLa cells, Jurkat cells
• Positive IHC detected in: mouse pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

25326-1-AP targets DYNC1LI1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DYNC1LI1 fusion protein Ag18191
• Full Name: dynein, cytoplasmic 1, light intermediate chain 1
• Calculated Molecular Weight: 523 aa, 57 kDa
• Observed Molecular Weight: 57-60 kDa
• GenBank Accession Number: BC131620
• Gene Symbol: DYNC1LI1
• Gene ID (NCBI): 51143
• RRID: AB_2880031
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Anal Chem; Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.; Authors - Hongwei Chu