DYNC1I1 Polyclonal antibody

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  WB analysis using 13808-1-AP

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 13808-1-AP (DYNC1I1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 13808-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13808-1-AP (DYNC1I1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 13808-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13808-1-AP (DYNC1I1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HUVEC using 13808-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using DYNC1I1 antibody (13808-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

DYNC1I1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 13808-1-AP

Publications(4)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

DNCIC1, DNCI1, DH IC-1

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Tested Applications

• Positive WB detected in: U-87 MG cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

13808-1-AP targets DYNC1I1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DYNC1I1 fusion protein Ag4810
• Full Name: dynein, cytoplasmic 1, intermediate chain 1
• Calculated Molecular Weight: 645 aa, 71 kDa
• Observed Molecular Weight: 71 kDa
• GenBank Accession Number: BC022540
• Gene Symbol: DYNC1I1
• Gene ID (NCBI): 1780
• RRID: AB_2093492
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O14576
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF,IHC	Oncogene; Cytoplasmic dynein regulates the subcellular localization of sphingosine kinase 2 to elicit tumor-suppressive functions in glioblastoma.; Authors - Heidi A Neubauer ; KD Validated
human	WB	Front Mol Neurosci; MFN2 Deficiency Impairs Mitochondrial Transport and Downregulates Motor Protein Expression in Human Spinal Motor Neurons; Authors - Yongchao Mou
mouse	WB	Neurobiol Dis; Pain sensitivity related to gamma oscillation of parvalbumin interneuron in primary somatosensory cortex in Dync1i1-/- mice; Authors - Zhongzhao Guo ; KO Validated
mouse	WB,IHC	Exp Gerontol; The potential benefits of PGC-1α in treating Alzheimer's disease are dependent on the integrity of the LLKYL L3 motif: Effect of regulating mitochondrial axonal transportation; Authors - Hou-Zhen Shi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Q (Verified Customer) (03-22-2021)

An OK antibody to detect DIC1, although the signal is a little weak and strong ECL is required.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: mouse primary neurons

DYNC1I1 Antibody Western Blot validation (1:500 dilution) in mouse primary neurons (Cat no:13808-1-AP)