CoraLite® Plus 750-conjugated DYKDDDDK tag Recombinant antibody (Binds to FLAG® tag epitope)

DYKDDDDK tag Uni-rAb^TM Recombinant Antibody for WB

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

4K14

Cat no : CL750-80010

Synonyms

DDDDK, Flag tag, Flag, DYKDDDDK, DDDDK tag

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Validation Data Gallery

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  WB analysis using CL750-80010

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-80010 (DYKDDDDK tag antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  FC experiment of Transfected HEK-293 using CL750-80010

  1x10^6 Transfected HEK-293 cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated DYKDDDDK tag Recombinant antibody (Binds to FLAG® tag epitope) (CL750-80010, Clone:4K14)(red). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: Recombinant protein
• Positive FC (Intra) detected in: Transfected HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL750-80010 targets DYKDDDDK tag in WB applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DYKDDDDK tag fusion protein Ag2329
• Full Name: DYKDDDDK tag
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DYKDDDDK Tag (Equivalent To FLAG Antibody From Sigma) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DYKDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.