CoraLite® Plus 750-conjugated DYKDDDDK tag Recombinant antibody (Binds to FLAG® tag epitope)

DYKDDDDK tag Recombinant Antibody for WB

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

4K14

Cat no : CL750-80010

Synonyms

DDDDK, DDDDK tag, DYKDDDDK, DYKDDDDK tag, Flag, Flag tag



Tested Applications

Positive WB detected inRecombinant protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL750-80010 targets DYKDDDDK tag in WB applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DYKDDDDK tag fusion protein Ag2329
Full Name DYKDDDDK tag
Gene Symbol
Gene ID (NCBI)
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DYKDDDDK Tag (Equivalent To FLAG Antibody From Sigma) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DYKDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

Protocols

Product Specific Protocols
WB protocol for CL Plus 750 DYKDDDDK tag antibody CL750-80010Download protocol
Standard Protocols
Click here to view our Standard Protocols