DYKDDDDK tag Recombinant antibody, PBS Only (Binds to FLAG® tag epitope)

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  IP experiment of Transfected HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

  IP result of anti-DYKDDDDK tag (IP:80010-1-RR, 4ug; Detection:80010-1-RR 1:3000) with Transfected HEK-293 cells lysate 400 ug. This data was developed using the same antibody clone with 80010-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 (eGFP-DYKDDDDK transfected) cells using DYKDDDDK tag antibody (80010-1-RR, Clone: 4K14 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), eGFP (Green), DYKDDDDK (Red), DAPI (Blue). This data was developed using the same antibody clone with 80010-1-PBS in a different storage buffer formulation.

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  IF Staining of Transfected HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Transfected HEK-293 cells using DYKDDDDK tag antibody (80010-1-RR, Clone: 4K14 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80010-1-PBS in a different storage buffer formulation.

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  IF Staining of Transfected HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using 80010-1-RR (DDDDK tag antibody), at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80010-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Transfected HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using 80010-1-RR (DYKDDDDK tag antibody), at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80010-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using DYKDDDDK tag antibody (80010-1-RR, Clone: 4K14 ) at dilution of 1:500 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), GFP (green). This data was developed using the same antibody clone with 80010-1-PBS in a different storage buffer formulation.

DYKDDDDK tag Uni-rAb^TM Recombinant Antibody for IF/ICC, IP, Indirect ELISA

Cat No. 80010-1-PBS

Clone No.4K14

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

IF/ICC, IP, Indirect ELISA

Synonyms

DDDDK, Flag tag, Flag, DYKDDDDK, DDDDK tag

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The antibody 80010-1-RR is suggested for IP and IF.

Product Information

80010-1-PBS targets DYKDDDDK tag in IF/ICC, IP, Indirect ELISA applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DYKDDDDK tag fusion protein Ag2329
• Full Name: Flag Tag
• Gene Symbol: Flag Tag
• Gene ID (NCBI): 8
• RRID: AB_2882940
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

DYKDDDDK Tag (Equivalent To FLAG Antibody From Sigma) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DYKDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.