DYKDDDDK tag Monoclonal antibody, PBS Only (Binds to FLAG® tag epitope)

DYKDDDDK tag Monoclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

recombinant protein

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B3C4

Cat no : 66008-3-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HEK-293 using 66008-3-Ig (same clone as 66008-3-PBS)

  HEK-293 and DDDDK-DPM1 transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 66008-3-Ig (DDDDK tag antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66008-3-PBS in a different storage buffer formulation.

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  IP experiment of Transfected HEK-293 using 66008-3-Ig (same clone as 66008-3-PBS)

  IP result of anti-DYKDDDDK tag (IP:66008-3-Ig, 5ug; Detection:66008-3-Ig 1:1000) with Transfected HEK-293 cells lysate 1600 ug. This data was developed using the same antibody clone with 66008-3-PBS in a different storage buffer formulation.

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  IF Staining of Transfected HEK-293 using 66008-3-Ig (same clone as 66008-3-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using 66008-3-Ig (DDDDK tag antibody) at dilution of 1:400 and Alexa Fluor 594-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66008-3-PBS in a different storage buffer formulation.

• 

  IF Staining of Transfected HEK-293 using 66008-3-Ig (same clone as 66008-3-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using 66008-3-Ig (DDDDK tag antibody) at dilution of 1:400 and Alexa Fluor 594-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66008-3-PBS in a different storage buffer formulation.

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Product Information

66008-3-PBS targets DYKDDDDK tag in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: DYKDDDDK tag
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

DYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DKYDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.