DUS3L Polyclonal antibody

• Featured Product
• KD/KO Validated

DUS3L Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15643-1-AP

Synonyms

DUS3, DUS3L

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Validation Data Gallery

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  WB analysis using 15643-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15643-1-AP (DUS3L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 15643-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15643-1-AP (DUS3L antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15643-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15643-1-AP (DUS3L antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, MCF-7 cells, PC-3 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Product Information

15643-1-AP targets DUS3L in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DUS3L fusion protein Ag8142
• Full Name: dihydrouridine synthase 3-like (S. cerevisiae)
• Calculated Molecular Weight: 46 kDa, 72 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: BC004549
• Gene Symbol: DUS3L
• Gene ID (NCBI): 56931
• RRID: AB_2293096
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	Nat Chem Biol; Activity-based RNA-modifying enzyme probing reveals DUS3L-mediated dihydrouridylation.; Authors - Wei Dai ; KD Validated