DSP Monoclonal antibody

DSP Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B10D4

Cat no : 68364-1-Ig

Synonyms

desmoplakin, DP, DPI, DPII, DSP

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Validation Data Gallery

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  WB analysis using 68364-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68364-1-Ig (DSP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 68364-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68364-1-Ig (DSP antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 68364-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68364-1-Ig (DSP antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 68364-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using DSP antibody (68364-1-Ig, Clone: 1B10D4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, A431 cells, MCF-7 cells, HEK-293 cells
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68364-1-Ig targets DSP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DSP fusion protein Ag30499
• Full Name: desmoplakin
• Calculated Molecular Weight: 2871 aa, 332 kDa
• Observed Molecular Weight: 240-330 kDa
• GenBank Accession Number: BC140802
• Gene Symbol: DSP
• Gene ID (NCBI): 1832
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The desmoplakin (DP) is the most abundant desmosomal proteins and is located at the cytoplasmic portion of the desmosomes which are specialized cell-cell adhesion structures abundant in tissues such as muscle and epidermis that are subjected to mechanical stress. Desmoplakin 1 and 2 (DP 1 and 2) are two splice variants sharing common C-terminal and N-terminal globular head and tail domains, but differ in the length of the rod domain that links them. This antibody detects both DP1 (280-330 kDa) and DP2 (240-260 kDa). (PMID: 16467215, 11781569)