DPEP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

DPEP1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, FC, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 12222-1-AP

Synonyms

Dehydropeptidase I, Dipeptidase 1, dipeptidase 1 (renal), DPEP1, hRDP, MBD1, MDP, Microsomal dipeptidase, RDP, Renal dipeptidase

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Validation Data Gallery

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  WB analysis using 12222-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12222-1-AP (DPEP1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 12222-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12222-1-AP (DPEP1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse liver tissue, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• FC: See 1 publications below

Product Information

12222-1-AP targets DPEP1 in WB, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DPEP1 fusion protein Ag2907
• Full Name: dipeptidase 1 (renal)
• Calculated Molecular Weight: 411 aa, 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC017023
• Gene Symbol: DPEP1
• Gene ID (NCBI): 1800
• RRID: AB_2877837
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DPEP1(Dipeptidase 1) is also named as MDP, RDP and belongs to the peptidase M19 family. It is a glycoprotein that plays an important role in the regulation of leukotriene activity. DPEP1 converts lekotriene D4 to leukotriene E4.

Publications

Species	Application	Title
mouse,human	WB	Cell; Dipeptidase-1 Is an Adhesion Receptor for Neutrophil Recruitment in Lungs and Liver.; Authors - Saurav Roy Choudhury ; KO Validated
human	WB,FC	Sci Adv; Dexamethasone sensitizes to ferroptosis by glucocorticoid receptor-induced dipeptidase-1 expression and glutathione depletion.; Authors - Anne von Mässenhausen ; KD Validated
human	IHC	Sci Adv; Dipeptidase-1 governs renal inflammation during ischemia reperfusion injury.; Authors - Arthur Lau
monkey		J Clin Invest; Renal immune surveillance and dipeptidase-1 contribute to contrast-induced acute kidney injury.; Authors - Arthur Lau
mouse	WB	Nat Commun; A single genetic locus controls both expression of DPEP1/CHMP1A and kidney disease development via ferroptosis.; Authors - Yuting Guan ; KO Validated
mouse,human	WB	Int Immunopharmacol; Dipeptidase 1 promotes ferroptosis in renal tubular epithelial cells in diabetic nephropathy via inhibition of the GSH/GPX4 axis; Authors - Yan Li