DOT1L Polyclonal antibody

DOT1L Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28145-1-AP

Synonyms

Lysine N methyltransferase 4, KMT4, KIAA1814, H3 K79 HMTase, DOT1 like protein

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Validation Data Gallery

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  WB analysis using 28145-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28145-1-AP (DOT1L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: PC-12 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28145-1-AP targets DOT1L in WB, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DOT1L fusion protein Ag27229
• Full Name: DOT1-like, histone H3 methyltransferase (S. cerevisiae)
• Calculated Molecular Weight: 185 kDa
• Observed Molecular Weight: 185~200 kDa
• GenBank Accession Number: NM_032482
• Gene Symbol: DOT1L
• Gene ID (NCBI): 84444
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DOT1L (Disruptor of telomeric silencing 1-like) is the only known histone methyltransferse catalyzing H3K79 methylation which is an activating mark for gene transcription by promoting transcriptional elongation (PMID: 34187895). DOT1L forms a complex with AF10/AF17 and ENL/AF9, is dysregulated in mixed-lineage leukemia (MLLr), and is believed to regulate transcriptional elongation without direct evidence. DOT1L has been reported to directly interact with RNAPII phosphorylated on Ser2 and/or Ser5 of the C-terminal domain (CTD) of its largest subunit. Loss of Dot1L results in yolk sac angiogenesis defects and embryonic lethality (PMID: 18787701).