DOCK2 Monoclonal antibody

• Featured Product
• KD/KO Validated

DOCK2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1D7A8

Cat no : 66969-1-Ig

Synonyms

dedicator of cytokinesis 2, DOCK2, FLJ46592, KIAA0209

---

Validation Data Gallery

• 

  WB analysis of Jurkat using 66969-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66969-1-Ig (DOCK2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Ramos using 66969-1-Ig

  Ramos cells were subjected to SDS PAGE followed by western blot with 66969-1-Ig (DOCK2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66969-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66969-1-Ig (DOCK2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 66969-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66969-1-Ig (DOCK2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66969-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66969-1-Ig (DOCK2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66969-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DOCK2 antibody (66969-1-Ig, Clone: 1D7A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

---

Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, Ramos cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

66969-1-Ig targets DOCK2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DOCK2 fusion protein Ag28582
• Full Name: dedicator of cytokinesis 2
• Calculated Molecular Weight: 212 kDa, 38 kDa
• Observed Molecular Weight: 200-210 kDa
• GenBank Accession Number: BC016996
• Gene Symbol: DOCK2
• Gene ID (NCBI): 1794
• RRID: AB_2882291
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DOCK2, also named as KIAA0209, belongs to the DOCK family. It is involved in cytoskeletal rearrangements required for lymphocyte migration in response of chemokines. DOCK2 activates RAC1 and RAC2 small GTPases, probably by functioning as a guanine nucleotide exchange factor (GEF), which exchanges bound GDP for free GTP. DOCK2 may also participate in IL2 transcriptional activation via the activation of RAC2. The antibody is specific to DOCK2.

Publications

Species	Application	Title
human	WB	Stem Cell Res Ther; Requirement of splicing factor hnRNP A2B1 for tumorigenesis of melanoma stem cells.; Authors - Mengqi Chu ; KD Validated