DNMT3B Polyclonal antibody

• Featured Product
• KD/KO Validated

DNMT3B Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

21

Cat no : 26971-1-AP

Synonyms

ICF, EC:2.1.1.37, DNMT3, DNMT, DNA (cytosine-5)-methyltransferase 3B

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Validation Data Gallery

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  WB analysis using 26971-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26971-1-AP (DNMT3B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26971-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26971-1-AP (DNMT3B antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 26971-1-AP

  IP result of anti-DNMT3B (IP:26971-1-AP, 4ug; Detection:26971-1-AP 1:5000) with K-562 cells lysate 1800 ug.

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  IHC staining of mouse brain using 26971-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 26971-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 26971-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 26971-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 26971-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 26971-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells, MCF-7 cells, Jurkat cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: mouse brain tissue, human breast cancer tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 20 publications below
• IHC: See 5 publications below
• IF: See 5 publications below
• CoIP: See 2 publications below
• ChIP: See 1 publications below

Product Information

26971-1-AP targets DNMT3B in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DNMT3B fusion protein Ag25117
• Full Name: DNA (cytosine-5-)-methyltransferase 3 beta
• Calculated Molecular Weight: 95 kDa
• Observed Molecular Weight: 85-96 kDa
• GenBank Accession Number: NM_001207055
• Gene Symbol: DNMT3B
• Gene ID (NCBI): 1789
• RRID: AB_2880705
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNMT3B is a DNA-(cytosine C5)-methyltransferase that is responsible for establishing the methylation patterns in cooperation with DNMT3A through the de novo methylation pathway. It is essential for the establishment of DNA methylation patterns during development. DNMT3B contains a C-terminal catalytic domain and an N-terminal regulatory domain, which are involved in targeting chromatin to regulate its function (PMID: 31547729). DNMT3B has 8 isoforms with the molecular mass of 80-96 kDa.

Publications

Species	Application	Title
human,mouse	IF,WB	Protein Cell; The chemical reprogramming of unipotent adult germ cells towards authentic pluripotency and de novo establishment of imprinting; Authors - Yuhan Chen
human	WB	Front Cell Dev Biol; miR-29b-3p Increases Radiosensitivity in Stemness Cancer Cells via Modulating Oncogenes Axis; Authors - Dong Pan
rat	IHC	Andrology; Methylation of eNOS in the rat penile corpus cavernosum under different pathological states and its relationship with erectile function; Authors - Na Wang
human	WB	Brain Sci; Neuroprotective Effect of Piracetam against Cocaine-Induced Neuro Epigenetic Modification of DNA Methylation in Astrocytes.; Authors - Kalaiselvi Sivalingam
human	WB	Biol Res; DNMT1-mediated PPARα methylation aggravates damage of retinal tissues in diabetic retinopathy mice.; Authors - Ying Zhu
human	WB	Integr Cancer Ther; Huaier Inhibits Proliferation, Migration, and Invasion of Cutaneous Squamous Cell Carcinoma Cells by Inhibiting the Methylation Levels of CDKN2A and TP53.; Authors - Liang Wang