DNMT3B Polyclonal antibody

DNMT3B Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (1)





Cat no : 26971-1-AP


DNA methyltransferase HsaIIIB, DNA MTase HsaIIIB, DNMT3B, ICF, M.HsaIIIB

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, MCF-7 cells, K-562 cells
Positive IHC detected inmouse brain tissue, human breast cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 26971-1-AP is DNMT3B Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, goat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNMT3B fusion protein Ag25117
Full Name DNA (cytosine-5-)-methyltransferase 3 beta
Calculated molecular weight 95 kDa
Observed molecular weight 85-96 kDa
GenBank accession numberNM_001207055
Gene symbol DNMT3B
Gene ID (NCBI) 1789
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DNMT3B is a DNA-(cytosine C5)-methyltransferase that is responsible for establishing the methylation patterns in cooperation with DNMT3A through the de novo methylation pathway. It is essential for the establishment of DNA methylation patterns during development. DNMT3B contains a C-terminal catalytic domain and an N-terminal regulatory domain, which are involved in targeting chromatin to regulate its function (PMID: 31547729). DNMT3B has 8 isoforms with the molecular mass of 80-96 kDa.


Product Specific Protocols
WB protocol for DNMT3B antibody 26971-1-APDownload protocol
IHC protocol for DNMT3B antibody 26971-1-APDownload protocol
IF protocol for DNMT3B antibody 26971-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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Epigenetic modification differences between fetal fibroblast cells and mesenchymal stem cells of the Arbas Cashmere goat.

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DNMT1-mediated PPARα methylation aggravates damage of retinal tissues in diabetic retinopathy mice.

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Brain Sci

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