DNMT3A Polyclonal antibody

• Featured Product
• KD/KO Validated

DNMT3A Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 19366-1-AP

Synonyms

DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, DNMT3A, DNMT3A2, M.HsaIIIA

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Validation Data Gallery

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  IHC staining of human kidney using 19366-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19366-1-AP (DNMT3A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 19366-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19366-1-AP (DNMT3A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 19366-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 19366-1-AP (DNMT3A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 19366-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 19366-1-AP (DNMT3A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 19366-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19366-1-AP (DNMT3A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 19366-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19366-1-AP (DNMT3A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 19366-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19366-1-AP (DNMT3A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 19366-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19366-1-AP (DNMT3A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human kidney tissue, human lung cancer tissue, mouse heart tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below

Product Information

The immunogen of 19366-1-AP is DNMT3A Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DNMT3A fusion protein Ag7015
• Full Name: DNA (cytosine-5-)-methyltransferase 3 alpha
• Calculated Molecular Weight: 912 aa, 102 kDa
• GenBank Accession Number: BC043617
• Gene Symbol: DNMT3A
• Gene ID (NCBI): 1788
• RRID: AB_10642139
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNA methylation in vertebrate animals is an epigenetic modification that is important for embryonic development, imprinting, and the inactivation of X chromosomes. DNA methylation is catalyzed by a family of DNA methyltransferases (DNMTs) that include the maintenance enzyme DNMT1 and de novo methyltransferases DNMT3a and DNMT3b. The overexpression of DNMT1, DNMT3a, and DNMT3b has been reported in various malignancies, including gastric, urothelial, and lung cancers, and may be related to tumorigenesis, tumor progression, and poor survival. Two isoforms of DNMT3a exist: the full-length DNMT3a, and the shorter form DNMT3a2 which lacks the N-terminal fragment. DNMT3a is expressed ubiquitously at low levels, while DNMT3a2 is specially expressed at high levels in embryonic stem cells and shows restricted expression in tissues known to undergo de novo methylation including testis and ovary. This antibody was raised against the N-terminal region of human DNMT3a. It is expected to detect the 120-130 kDa DNMT3a but not 72-100 kDa DNMT3a2.

Publications

Species	Application	Title
mouse	WB	Nucleic Acids Res; YY1 safeguard multidimensional epigenetic landscape associated with extended pluripotency.; Authors - Xiaotao Dong
mouse	WB	Development; RNA ligase RTCB regulates mRNA Alternative splicing and is required for mouse oocyte development and maintenance; Authors - Hua Zhang
human	WB	Aging (Albany NY); ACADS acts as a potential methylation biomarker associated with the proliferation and metastasis of hepatocellular carcinomas.; Authors - Diyu Chen
human	WB	Biochim Biophys Acta; HIC1 epigenetically represses CIITA transcription in B lymphocytes.; Authors - Sheng Zeng ; KD Validated
human	WB	J Appl Toxicol; Protein profiles of cardiomyocyte differentiation in murine embryonic stem cells exposed to perfluorooctane sulfonate.; Authors - Ying-Ying Zhang
human	WB	Int J Med Sci; Simultaneous silencing Aurora-A and UHRF1 inhibits colorectal cancer cell growth through regulating expression of DNMT1 and STAT1.; Authors - Jing Han