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DNMT3A Polyclonal antibody

DNMT3A Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19366-1-AP

Synonyms

DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, DNMT3A, DNMT3A2, M.HsaIIIA



Tested Applications

Positive IHC detected inhuman kidney tissue, human lung cancer tissue, mouse heart tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 19366-1-AP is DNMT3A Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNMT3A fusion protein Ag7015
Full Name DNA (cytosine-5-)-methyltransferase 3 alpha
Calculated molecular weight 912 aa, 102 kDa
GenBank accession numberBC043617
Gene symbol DNMT3A
Gene ID (NCBI) 1788
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DNA methylation in vertebrate animals is an epigenetic modification that is important for embryonic development, imprinting, and the inactivation of X chromosomes. DNA methylation is catalyzed by a family of DNA methyltransferases (DNMTs) that include the maintenance enzyme DNMT1 and de novo methyltransferases DNMT3a and DNMT3b. The overexpression of DNMT1, DNMT3a, and DNMT3b has been reported in various malignancies, including gastric, urothelial, and lung cancers, and may be related to tumorigenesis, tumor progression, and poor survival. Two isoforms of DNMT3a exist: the full-length DNMT3a, and the shorter form DNMT3a2 which lacks the N-terminal fragment. DNMT3a is expressed ubiquitously at low levels, while DNMT3a2 is specially expressed at high levels in embryonic stem cells and shows restricted expression in tissues known to undergo de novo methylation including testis and ovary. This antibody was raised against the N-terminal region of human DNMT3a. It is expected to detect the 120-130 kDa DNMT3a but not 72-100 kDa DNMT3a2.

Protocols

Product Specific Protocols
IHC protocol for DNMT3A antibody 19366-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Med Sci

Simultaneous silencing Aurora-A and UHRF1 inhibits colorectal cancer cell growth through regulating expression of DNMT1 and STAT1.

Authors - Jing Han
humanWB

J Appl Toxicol

Protein profiles of cardiomyocyte differentiation in murine embryonic stem cells exposed to perfluorooctane sulfonate.

Authors - Ying-Ying Zhang
humanWB

Biochim Biophys Acta

HIC1 epigenetically represses CIITA transcription in B lymphocytes.

Authors - Sheng Zeng
  • KD Validated
humanWB

Aging (Albany NY)

ACADS acts as a potential methylation biomarker associated with the proliferation and metastasis of hepatocellular carcinomas.

Authors - Diyu Chen
mouseWB

Nucleic Acids Res

YY1 safeguard multidimensional epigenetic landscape associated with extended pluripotency.

Authors - Xiaotao Dong