DNMT2 Polyclonal antibody

• Featured Product
• KD/KO Validated

DNMT2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 19221-1-AP

Synonyms

DNA MTase homolog HsaIIP, DNMT2, M.HsaIIP, PuMet, RNMT1, TRDMT1

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Validation Data Gallery

• 

  WB analysis of HeLa using 19221-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19221-1-AP (DNMT2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 19221-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 19221-1-AP (DNMT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 19221-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19221-1-AP (DNMT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 19221-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19221-1-AP (DNMT2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 19221-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19221-1-AP (DNMT2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, MCF-7 cells, mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Product Information

19221-1-AP targets DNMT2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DNMT2 fusion protein Ag5571
• Full Name: tRNA aspartic acid methyltransferase 1
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 43-45 kDa
• GenBank Accession Number: BC047733
• Gene Symbol: DNMT2
• Gene ID (NCBI): 1787
• RRID: AB_10597075
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Dnmt2 was initially assigned a member of the DNA methyltransferase family on the basis of its extensive homology with eukaryotic and prokaryotic DNA-(cytosine C5)-methyltransferases . However, in the apparent absence of a phenotype in dnmt2 knockout cells, Dnmt2's possible biological function remained unknown , even though Dnmt2 is strongly conserved and it is found in species ranging from Schizosaccharomyces pombe to human. Later very weak, residual DNA methylation activity was found with enzymes from different species. The finding that Dnmt2 is an active RNA methyltransferase capable of methylating the C38 position of the tRNA Asp came as a surprise . However, still no cellular function of the tRNA Asp methylation has been found, although in Zebrafish Dnmt2 knock-down caused a developmental phenotype . It is very intriguing that an enzyme that looks like a DNA methyltransferase can methylate RNA, in particular since the RNA and DNA specific m5 C methyltransferases use different catalytic residues and a different mechanism for the methyl transfer reaction . the predicted molecular weight of Dnmt2 is 45 kDa, but it may migrate to the 40 kDa (PMID: 18567810 )

Publications

Species	Application	Title
human	WB	Clin Transl Med; Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1.; Authors - Lijie Zhou
human	WB	BMC Biol; Multiple links between 5-methylcytosine content of mRNA and translation.; Authors - Ulrike Schumann ; KD Validated
human	WB,IF	Int J Biol Macromol; Restricted tRNA methylation by intermolecular disulfide bonds in DNMT2/TRDMT1; Authors - Huari Li
human	WB	Cell Death Dis; Epigenetic addition of m5C to HBV transcripts promotes viral replication and evasion of innate antiviral responses; Authors - Shuang Ding