Dynamin 1 Polyclonal antibody

• Featured Product
• KD/KO Validated

Dynamin 1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 18205-1-AP

Synonyms

DNM, DNM1, dynamin 1, Dynamin1

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Validation Data Gallery

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  WB analysis of mouse brain using 18205-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18205-1-AP (Dynamin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 18205-1-AP

  WB result of Dynamin 1 antibody (18205-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Dynamin 1 transfected A549 cells.

• 

  WB analysis of NIH/3T3 using 18205-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18205-1-AP (Dynamin 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 18205-1-AP

  IP result of anti-Dynamin 1 (IP:18205-1-AP, 4ug; Detection:18205-1-AP 1:2000) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of rat brain using 18205-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18205-1-AP (Dynamin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 18205-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18205-1-AP (Dynamin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18205-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18205-1-AP (Dynamin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18205-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18205-1-AP (Dynamin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Neuro-2a using 18205-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using 18205-1-AP (Dynamin 1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, NIH/3T3 cells, A549 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: rat brain tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Neuro-2a cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IF: See 1 publications below

Product Information

18205-1-AP targets Dynamin 1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig, chicken, fish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Dynamin 1 fusion protein Ag12827
• Full Name: dynamin 1
• Calculated Molecular Weight: 851 aa, 96 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC063850
• Gene Symbol: DNM1
• Gene ID (NCBI): 1759
• RRID: AB_2093197
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Elife; Amphiphysin I cleavage by asparagine endopeptidase leads to tau hyperphosphorylation and synaptic dysfunction.; Authors - Xingyu Zhang
mouse,pig	WB	PLoS Pathog; Pseudorabies virus inhibits progesterone-induced inactivation of TRPML1 to facilitate viral entry; Authors - Bing-Qian Su
fish	WB	Int J Mol Sci; Dietary Nano-ZnO Is Absorbed via Endocytosis and ZIP Pathways, Upregulates Lipogenesis, and Induces Lipotoxicity in the Intestine of Yellow Catfish.; Authors - Shu-Wei Chen
human	WB	Commun Biol; The germline factor DDX4 contributes to the chemoresistance of small cell lung cancer cells; Authors - Christopher Noyes ; KD Validated
human	WB	Neurobiol Dis; Asparagine endopeptidase cleaves synaptojanin 1 and triggers synaptic dysfunction in Parkinson's disease.; Authors - Li Zou
mouse	IF	J Neurosci; The Structural and Functional Integrity of Rod Photoreceptor Ribbon Synapses Depends on Redundant Actions of Dynamins 1 and 3; Authors - Christin Hanke-Gogokhia ; KD Validated