DLGAP3 Polyclonal antibody

• Featured Product
• KD/KO Validated

DLGAP3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 55056-1-AP

Synonyms

PSD-95/SAP90-binding protein 3, PSD 95/SAP90 binding protein 3, Disks large-associated protein 3, DAP-3, DAP3

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Validation Data Gallery

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  WB analysis of human brain using 55056-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55056-1-AP (DLGAP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 55056-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55056-1-AP (DLGAP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 55056-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 55056-1-AP (DLGAP3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human brain tissue, mouse brain tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Product Information

55056-1-AP targets DLGAP3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: discs, large (Drosophila) homolog-associated protein 3
• Calculated Molecular Weight: 106 kDa
• Observed Molecular Weight: 106-120 kDa
• GenBank Accession Number: NM_001080418
• Gene Symbol: DLGAP3
• Gene ID (NCBI): 58512
• RRID: AB_10858793
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DLGAP3, also named as DAP3, belongs to the SAPAP family. It may play a role in the molecular organization of synapses and neuronal cell signaling. DLGAP3 could be an adapter protein linking ion channel to the subsynaptic cytoskeleton. It may induce enrichment of PSD-95/SAP90 at the plasma membrane. The antibody has no cross reaction to other DLGAP members.

Publications

Species	Application	Title
rat	WB	Biol Psychiatry; Metabotropic Glutamate Receptor and Fragile X Signaling in a Female Model of Escalated Aggression.; Authors - Laura E Been
human,mouse	WB,IF	Cell Death Dis; SAPAP3 regulates epileptic seizures involving GluN2A in post-synaptic densities.; Authors - Yanke Zhang ; KD Validated
mouse	WB	Nat Neurosci; Neuronal activity rapidly reprograms dendritic translation via eIF4G2:uORF binding; Authors - Ezgi Hacisuleyman