SAP102 Polyclonal antibody

SAP102 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 18036-1-AP

Synonyms

Disks large homolog 3, DLG3, KIAA1232, MRX, MRX90, NE Dlg, NEDLG, Neuroendocrine DLG, SAP 102, SAP102, Synapse associated protein 102, XLMR

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Validation Data Gallery

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  WB analysis using 18036-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18036-1-AP (SAP102 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 18036-1-AP

  IP result of anti-SAP102 (IP:18036-1-AP, 3ug; Detection:18036-1-AP 1:3000) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse brain using 18036-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18036-1-AP (SAP102 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 18036-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18036-1-AP (SAP102 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

18036-1-AP targets SAP102 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SAP102 fusion protein Ag12658
• Full Name: discs, large homolog 3 (Drosophila)
• Calculated Molecular Weight: 817 aa, 90 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC093864
• Gene Symbol: SAP102
• Gene ID (NCBI): 1741
• RRID: AB_2092175
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Synapse-associated protein 102 (SAP102), also known as DLG3, is a MAGUK that is highly expressed early in development and mediates receptor trafficking during synaptogenesis. The importance of SAP102 function in brain development is demonstrated by the fact that SAP102 mutations have been identified in human patients with nonsyndromic X-linked mental retardation (XLMR).SAP102 has critical roles in early cortical synapse development.

Publications

Species	Application	Title
human	IHC	Clin Exp Pharmacol Physiol; High expression of DLG3 is associated with decreased survival from breast cancer.; Authors - Jie Liu
mouse	WB	J Nutr Biochem; Hippocampal proteomic changes in high-fat diet-induced obese mice associated with memory decline; Authors - Ping Lu