DHODH Polyclonal antibody

• Featured Product
• KD/KO Validated

DHODH Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF-P, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

71

Reviews (2)

Cat no : 14877-1-AP

Synonyms

PYRD, EC:1.3.5.2, Dihydroorotate oxidase, Dihydroorotate dehydrogenase (quinone), mitochondrial, dihydroorotate dehydrogenase

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Validation Data Gallery

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  WB analysis using 14877-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14877-1-AP (DHODH antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse spleen using 14877-1-AP

  IP result of anti-DHODH (IP:14877-1-AP, 4ug; Detection:14877-1-AP 1:1000) with mouse spleen tissue lysate 4000ug.

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  IHC staining of human breast cancer using 14877-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14877-1-AP (DHODH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 14877-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14877-1-AP (DHODH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse kidney using 14877-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using DHODH antibody (14877-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HEK-293T using 14877-1-AP

  1X10^6 HEK-293T cells were intracellularly stained with 0.2 ug Anti-Human DHODH (14877-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A2780 cells, MCF-7 cells, SKOV-3 cells, mouse heart tissue, mouse ovary tissue, mouse spleen tissue, rat spleen tissue
• Positive IP detected in: mouse spleen tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse kidney tissue
• Positive FC (Intra) detected in: HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 13 publications below
• WB: See 66 publications below
• IHC: See 5 publications below
• IF: See 2 publications below
• FC: See 1 publications below

Product Information

14877-1-AP targets DHODH in WB, IHC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, chicken, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DHODH fusion protein Ag6649
• Full Name: dihydroorotate dehydrogenase
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC065245
• Gene Symbol: DHODH
• Gene ID (NCBI): 1723
• RRID: AB_2091723
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DHODH(Dihydroorotate dehydrogenase) catalyzes the fourth enzymatic step in de novo pyrimidine biosynthesis.DHO dehydrogenase is a monofunctional protein which, in most eukaryotic organisms, is located on the outer surface of the inner mitochondrial membrane.Defects in DHODH are the cause of postaxial acrofacial dysostosis (POADS).

Publications

Species	Application	Title
human	WB	Cell Res; Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression; Authors - Shiqiao Qiu
human	WB	Nature; DHODH-mediated ferroptosis defence is a targetable vulnerability in cancer.; Authors - Chao Mao ; KO Validated
human,mouse	WB	Science; Stimulation of de novo pyrimidine synthesis by growth signaling through mTOR and S6K1.; Authors - Issam Ben-Sahra
human,mouse	WB	Cancer Discov; PTEN Regulates Glutamine Flux to Pyrimidine Synthesis and Sensitivity to Dihydroorotate Dehydrogenase Inhibition.; Authors - Deepti Mathur
mouse	IHC	Cancer Cell; Cancer-selective metabolic vulnerabilities in MYC-amplified medulloblastoma; Authors - William D Gwynne ; KO Validated
mouse	WB	Cell Metab; Macrophage-Released Pyrimidines Inhibit Gemcitabine Therapy in Pancreatic Cancer.; Authors - Christopher J Halbrook ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kristian (Verified Customer) (07-06-2021)

Paraffin embedded kidneys were stained for Dhodh (green) and DAPI (blue)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse kidney

DHODH Antibody Immunofluorescence validation (1:200 dilution) in Mouse kidney (Cat no:14877-1-AP)

FH

Daniel (Verified Customer) (04-16-2019)

Highly specific detection of both mouse and human DHODH.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human RCC4 cells (lane1), Mouse kidney (lane2)

DHODH Antibody Western Blot, validation (1:1000 dilution) in Human RCC4 cells (lane1), Mouse kidney (lane2) (Cat no:14877-1-AP)