Desmin Polyclonal antibody

Desmin Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 22205-1-AP

Synonyms

DES

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Validation Data Gallery

• 

  WB analysis of mouse heart using 22205-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 22205-1-AP (Desmin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 22205-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22205-1-AP (Desmin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 22205-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22205-1-AP (Desmin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 22205-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22205-1-AP (Desmin antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human normal colon using 22205-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 22205-1-AP (Desmin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 22205-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22205-1-AP (Desmin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 22205-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22205-1-AP (Desmin antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse heart using 22205-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22205-1-AP (Desmin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse heart using 22205-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22205-1-AP (Desmin antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse skeletal muscle tissue
• Positive IHC detected in: human normal colon, human heart tissue, human skeletal muscle tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

22205-1-AP targets Desmin in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Desmin fusion protein Ag17525
• Full Name: desmin
• Calculated Molecular Weight: 470 aa, 54 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC032116
• Gene Symbol: Desmin
• Gene ID (NCBI): 1674
• RRID: AB_2879027
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.

Publications

Species	Application	Title
human	WB	Int J Biol Sci; Ursolic Acid Alleviates Mitotic Catastrophe in Podocyte by Inhibiting Autophagic P62 Accumulation in Diabetic Nephropathy; Authors - Hang Mei