RIG-1/DDX58 Polyclonal antibody
RIG-1/DDX58 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 25068-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A431 cells, HeLa cells, THP-1 cells, NIH/3T3 cells |
Positive IP detected in | A431 cells |
Positive IHC detected in | human heart tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 1 publications below |
IHC | See 1 publications below |
Product Information
25068-1-AP targets RIG-1/DDX58 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | RIG-1/DDX58 fusion protein Ag18585 |
Full Name | DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 |
Calculated molecular weight | 925 aa, 107 kDa |
Observed molecular weight | 101/106 kDa |
GenBank accession number | BC132786 |
Gene symbol | DDX58 |
Gene ID (NCBI) | 23586 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DDX58, also named as RIG-1, belongs to the helicase family. It is involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). It is essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for RIG-1/DDX58 antibody 25068-1-AP | Download protocol |
IHC protocol for RIG-1/DDX58 antibody 25068-1-AP | Download protocol |
IP protocol for RIG-1/DDX58 antibody 25068-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cancer Med RIG-I promotes IFN/JAK2 expression and the endoplasmic reticulum stress response to inhibit chemoradiation resistance in nasopharyngeal carcinoma. |