RIG-1/DDX58 Polyclonal antibody

RIG-1/DDX58 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 25068-1-AP

Synonyms

DDX58, RIG-I, Retinoic acid-inducible gene 1 protein, EC:3.6.4.13, DEAD box protein 58

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Validation Data Gallery

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  WB analysis using 25068-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25068-1-AP (RIG-1/DDX58 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 25068-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25068-1-AP (RIG-1/DDX58 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 25068-1-AP

  IP result of anti-RIG-1/DDX58 (IP:25068-1-AP, 4ug; Detection:25068-1-AP 1:600) with A431 cells lysate 1200ug.

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  IHC staining of human heart using 25068-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25068-1-AP (DDX58 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 25068-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25068-1-AP (DDX58 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 25068-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 25068-1-AP (RIG-1/DDX58 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 25068-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 25068-1-AP (RIG-1/DDX58 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, NIH/3T3 cells, THP-1 cells
• Positive IP detected in: A431 cells
• Positive IHC detected in: human colon tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below
• IP: See 2 publications below

Product Information

25068-1-AP targets RIG-1/DDX58 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RIG-1/DDX58 fusion protein Ag18585
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
• Calculated Molecular Weight: 925 aa, 106 kDa
• Observed Molecular Weight: 101~106 kDa
• GenBank Accession Number: BC132786
• Gene Symbol: RIG-1/DDX58
• Gene ID (NCBI): 23586
• RRID: AB_2879881
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DDX58, also named as RIG-1, belongs to the helicase family. It is involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). It is essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV.

Publications

Species	Application	Title
human	WB,IHC	Cancer Med; RIG-I promotes IFN/JAK2 expression and the endoplasmic reticulum stress response to inhibit chemoradiation resistance in nasopharyngeal carcinoma.; Authors - Di Jing
	WB	bioRxiv; A tale of two tumors: differential, but detrimental, effects of glioblastoma extracellular vesicles (EVs) on normal human brain cells; Authors - Mary Wang
human	WB,IP	Proc Natl Acad Sci U S A; IFIH1 (MDA5) is required for innate immune detection of intron-containing RNA expressed from the HIV-1 provirus; Authors - Mehmet Hakan Guney
human	WB,IP	bioRxiv; IFIH1 (MDA5) is required for innate immune detection of intron-containing RNA expressed from the HIV-1 provirus; Authors - Mehmet Hakan Guney