DDOST Polyclonal antibody

• Featured Product
• KD/KO Validated

DDOST Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 14916-1-AP

Synonyms

AGE R1, DDOST, DDOST 48 kDa subunit, KIAA0115, OK/SW cl.45, OST, OST48, WBP1

---

Validation Data Gallery

• 

  WB analysis of HeLa using 14916-1-AP

  WB result of DDOST antibody (14916-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDOST transfected HeLa cells.

• 

  WB analysis of A549 using 14916-1-AP

  WB result of DDOST antibody (14916-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDOST transfected A549 cells.

• 

  WB analysis using 14916-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14916-1-AP (DDOST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 14916-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14916-1-AP (DDOST antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 14916-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14916-1-AP (DDOST antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 14916-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 14916-1-AP (DDOST antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of rat small intestine using 14916-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 14916-1-AP (DDOST antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse pancreas using 14916-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 14916-1-AP (DDOST antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse pancreas using 14916-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 14916-1-AP (DDOST antibody) at dilution of 1:200 (under 40x lens).

---

Tested Applications

• Positive WB detected in: U-87 MG cells, A549 cells, HeLa cells, mouse pancreas, rat pancreas
• Positive IHC detected in: mouse testis tissue, rat small intestine tissue, mouse pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below
• IHC: See 2 publications below
• CoIP: See 1 publications below

Product Information

14916-1-AP targets DDOST in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DDOST fusion protein Ag6727
• Full Name: dolichyl-diphosphooligosaccharide-protein glycosyltransferase
• Calculated molecular weight: 49 kDa
• Observed molecular weight: 48 kDa
• GenBank accession number: BC002594
• Gene symbol: DDOST
• Gene ID (NCBI): 1650
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nat Chem Biol; Oligosaccharyltransferase inhibition induces senescence in RTK-driven tumor cells.; Authors - Cecilia Lopez-Sambrooks
mouse	WB,CoIP	Res Sq; Hexokinase-1 mitochondrial dissociation and protein O-GlcNAcylation drive heart failure with preserved ejection fraction; Authors - Hossein Ardehali
human	IHC	IET Syst Biol; An immune-related multi-omics analysis of dolichyl-diphosphooligosaccharide protein glycosyltransferase in glioma: Prognostic value exploration and competitive endogenous RNA network identification; Authors - Jie Liu
human	IHC	J Hepatocell Carcinoma; Identification of a Novel Prognostic Signature Based on N-Linked Glycosylation and Its Correlation with Immunotherapy Response in Hepatocellular Carcinoma; Authors - Shusheng Lin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Nicole (Verified Customer) (04-28-2022)

80µl RIPA Hela cell extract: Nitrocellulose: no signal PVDF: weak signal, double band

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hela