DDB1 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

DDB1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11380-1-PBS

Synonyms

UV DDB1, HBV X-associated protein 1, DNA damage-binding protein a, DNA damage-binding protein 1, DDBA

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Validation Data Gallery

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  WB analysis using 11380-1-AP (same clone as 11380-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11380-1-AP (DDB1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11380-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 11380-1-AP (same clone as 11380-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11380-1-AP (DDB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11380-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human kidney using 11380-1-AP (same clone as 11380-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11380-1-AP (DDB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11380-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 11380-1-AP (same clone as 11380-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11380-1-AP (DDB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11380-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat testis using 11380-1-AP (same clone as 11380-1-PBS)

  rat testis tissue were subjected to SDS PAGE followed by western blot with 11380-1-AP (DDB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11380-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 11380-1-AP (same clone as 11380-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11380-1-AP (DDB1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11380-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 11380-1-AP (same clone as 11380-1-PBS)

  IP result of anti-DDB1 (IP:11380-1-AP, 5ug; Detection:11380-1-AP 1:500) with Jurkat cells lysate 3080ug. This data was developed using the same antibody clone with 11380-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11380-1-AP (same clone as 11380-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11380-1-AP (DDB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11380-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11380-1-AP (same clone as 11380-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11380-1-AP (DDB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11380-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11380-1-AP (same clone as 11380-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 11380-1-AP (DDB1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11380-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11380-1-AP (same clone as 11380-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11380-1-AP (DDB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11380-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11380-1-AP (same clone as 11380-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11380-1-AP (DDB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11380-1-PBS in a different storage buffer formulation.

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Product Information

11380-1-PBS targets DDB1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DDB1 fusion protein Ag1901
• Full Name: damage-specific DNA binding protein 1, 127kDa
• Calculated Molecular Weight: 1140 aa, 127 kDa
• Observed Molecular Weight: 127 kDa
• GenBank Accession Number: BC011686
• Gene Symbol: DDB1
• Gene ID (NCBI): 1642
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

DDB1, also named as XAP1, XPCe, DDBa and XPE-BF, belongs to the DDB1 family. It is required for DNA repair. DDB1 binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. This antibody is specific to DDB1.