DCX Polyclonal antibody, PBS Only

DCX Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13925-1-PBS

Synonyms

Lis-X, Lissencephalin-X, Doublin, doublecortin

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Validation Data Gallery

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  WB analysis using 13925-1-AP (same clone as 13925-1-PBS)

  Fetal human brain lysates were subjected to SDS PAGE followed by western blot with 13925-1-AP (DCX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13925-1-PBS in a different storage buffer formulation.

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  WB analysis using 13925-1-AP (same clone as 13925-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13925-1-AP (DCX antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13925-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 13925-1-AP (same clone as 13925-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13925-1-AP (DCX antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13925-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 13925-1-AP (same clone as 13925-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13925-1-AP (DCX antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13925-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 13925-1-AP (same clone as 13925-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13925-1-AP (DCX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13925-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 13925-1-AP (same clone as 13925-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using DCX antibody (13925-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13925-1-PBS in a different storage buffer formulation.

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Product Information

13925-1-PBS targets DCX in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DCX fusion protein Ag4945
• Full Name: doublecortin
• Calculated Molecular Weight: 402 aa, 45 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC027925
• Gene Symbol: DCX
• Gene ID (NCBI): 1641
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

DCX (Doublecortin) is a microtubule-associated protein that is essential for normal brain development. DCX is expressed in migrating neurons throughout the central and peripheral nervous system during embryonic and postnatal development. Anti-DCX antibodies are widely used for detection of neurogenesis. It's also a common neuron marker since its expression is specific to neurons.