DCT Polyclonal antibody

DCT Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13095-1-AP

Synonyms

DCT, DT, L dopachrome Delta isomerase, L dopachrome tautomerase, TRP 2, TRP2, Tyrosinase related protein 2, TYRP2



Tested Applications

Positive WB detected inHL-60 cells, A375 cells, A431 cells, COS-7 cells, MCF-7 cells, rat skin tissue
Positive IP detected inHL-60 cells
Positive IHC detected inhuman skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

13095-1-AP targets DCT in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity human, mouse, rat, monkey
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DCT fusion protein Ag3714
Full Name dopachrome tautomerase (dopachrome delta-isomerase, tyrosine-related protein 2)
Calculated molecular weight 519 aa, 59 kDa
Observed molecular weight 59 kDa
GenBank accession numberBC028311
Gene symbol DCT
Gene ID (NCBI) 1638
RRIDAB_2090576
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DCT(L-dopachrome Delta-isomerase) is also named as TYRP2,TRP2 and belongs to the tyrosinase family.human TYRP2 protein has 83% identity and 90% similarity to the mouse sequence and has all the structural characteristics of the tyrosinase protein family, including a signal peptide, 15 conserved cysteine residues, 2 copper-binding domains, and a C-terminal membrane-spanning region(PMID:8206391).

Protocols

Product Specific Protocols
WB protocol for DCT antibody 13095-1-APDownload protocol
IHC protocol for DCT antibody 13095-1-APDownload protocol
IF protocol for DCT antibody 13095-1-APDownload protocol
IP protocol for DCT antibody 13095-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Curr Biol

Neuropsin (OPN5) Mediates Local Light-Dependent Induction of Circadian Clock Genes and Circadian Photoentrainment in Exposed Murine Skin.

Authors - Ethan D Buhr
humanIHC

Hum Mol Genet

Transcriptome analysis and molecular signature of human retinal pigment epithelium.

Authors - Strunnikova N V NV
humanWB

J Invest Dermatol

miR-137 inhibits the invasion of melanoma cells through downregulation of multiple oncogenic target genes.

Authors - Chonglin Luo
humanWB

J Cell Mol Med

Kaempferol promotes melanogenesis and reduces oxidative stress in PIG1 normal human skin melanocytes

Authors - Yihui Xie
humanWB

Sci Rep

Loss of tumorigenic potential upon transdifferentiation from keratinocytic into melanocytic lineage.

Authors - Sabrina Fehrenbach
mouseWB

Pharmaceutics

Anti-Melanogenic Mechanism of Tetrahydrocurcumin and Enhancing Its Topical Delivery Efficacy Using a Lecithin-Based Nanoemulsion.

Authors - Xudong Tang