DCT Polyclonal antibody

DCT Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

16

Cat no : 13095-1-AP

Synonyms

TRP2, TRP 2, L-dopachrome tautomerase, L-dopachrome Delta-isomerase, EC:5.3.3.12

---

Validation Data Gallery

• 

  WB analysis of HL-60 using 13095-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13095-1-AP (DCT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COS-7 using 13095-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 13095-1-AP (DCT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 13095-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13095-1-AP (DCT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 13095-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13095-1-AP (DCT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 13095-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13095-1-AP (DCT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 13095-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 13095-1-AP (DCT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 13095-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 13095-1-AP (DCT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skin using 13095-1-AP

  rat skin tissue were subjected to SDS PAGE followed by western blot with 13095-1-AP (DCT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 13095-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 13095-1-AP (DCT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HL-60 using 13095-1-AP

  IP result of anti-DCT (IP:13095-1-AP, 4ug; Detection:13095-1-AP 1:500) with HL-60 cells lysate 4000ug.

• 

  IHC staining of human skin using 13095-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 13095-1-AP (DCT antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 13095-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 13095-1-AP (DCT antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of A549 using 13095-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using DCT antibody (13095-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HL-60 cells, A375 cells, A431 cells, COS-7 cells, MCF-7 cells, rat skin tissue
• Positive IP detected in: HL-60 cells
• Positive IHC detected in: human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 12 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

13095-1-AP targets DCT in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

• Tested Reactivity: human, mouse, rat, monkey
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DCT fusion protein Ag3714
• Full Name: dopachrome tautomerase (dopachrome delta-isomerase, tyrosine-related protein 2)
• Calculated Molecular Weight: 519 aa, 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC028311
• Gene Symbol: DCT
• Gene ID (NCBI): 1638
• RRID: AB_2090576
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DCT(L-dopachrome Delta-isomerase) is also named as TYRP2,TRP2 and belongs to the tyrosinase family.human TYRP2 protein has 83% identity and 90% similarity to the mouse sequence and has all the structural characteristics of the tyrosinase protein family, including a signal peptide, 15 conserved cysteine residues, 2 copper-binding domains, and a C-terminal membrane-spanning region(PMID:8206391).

Publications

Species	Application	Title
mouse	IF	Curr Biol; Neuropsin (OPN5) Mediates Local Light-Dependent Induction of Circadian Clock Genes and Circadian Photoentrainment in Exposed Murine Skin.; Authors - Ethan D Buhr
human	IHC	Hum Mol Genet; Transcriptome analysis and molecular signature of human retinal pigment epithelium.; Authors - Strunnikova N V NV
human	WB	J Invest Dermatol; miR-137 inhibits the invasion of melanoma cells through downregulation of multiple oncogenic target genes.; Authors - Chonglin Luo
human	WB	J Cell Mol Med; Kaempferol promotes melanogenesis and reduces oxidative stress in PIG1 normal human skin melanocytes; Authors - Yihui Xie
human	WB	Sci Rep; Loss of tumorigenic potential upon transdifferentiation from keratinocytic into melanocytic lineage.; Authors - Sabrina Fehrenbach
mouse	WB	Pharmaceutics; Anti-Melanogenic Mechanism of Tetrahydrocurcumin and Enhancing Its Topical Delivery Efficacy Using a Lecithin-Based Nanoemulsion.; Authors - Xudong Tang