DCLRE1B Polyclonal antibody

DCLRE1B Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13203-1-AP

Synonyms

EC:3.1.-.-, DNA cross-link repair 1B protein, Beta-lactamase DCLRE1B, APOLLO, 5' exonuclease Apollo

---

Validation Data Gallery

• 

  WB analysis of Jurkat using 13203-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13203-1-AP (DCLRE1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13203-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13203-1-AP (DCLRE1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 13203-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 13203-1-AP (DCLRE1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: Jurkat cells, HepG2 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

13203-1-AP targets DCLRE1B in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DCLRE1B fusion protein Ag3945
• Full Name: DNA cross-link repair 1B (PSO2 homolog, S. cerevisiae)
• Calculated Molecular Weight: 532 aa, 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC029687
• Gene Symbol: DCLRE1B
• Gene ID (NCBI): 64858
• RRID: AB_2292695
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interstrand cross-links (ICLs) is an extremely cytotoxic form of DNA damage that covalently tethers the two strands of the double helix, which inhibits fundamental nuclear functions, including transcription and DNA replication [PMID:21701511]. DCLRE1B is one of several evolutionarily conserved genes involved in repair of interstrand cross-links [PMID:10848582]. Furthermore, DCLRE1B was identified as a TRF2-interacting protein and, has a role in telomere maintenance in association with the Shelterin complex, preventing non-homologous end-joining-mediated chromosome fusions protecting leading strand telomeres [PMID:20619712].

Publications

Species	Application	Title
human	WB	Nat Struct Mol Biol; Fanconi anemia DNA crosslink repair factors protect against LINE-1 retrotransposition during mouse development; Authors - Nazareno Bona