Cytokeratin 8 Polyclonal antibody

Cytokeratin 8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 27105-1-AP

Synonyms

Cytokeratin 8-specific, KRT8, Cytokeratin,Keratin, CK-8, CK8

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Validation Data Gallery

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  WB analysis using 27105-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 27105-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 27105-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 27105-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 27105-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brown disease using 27105-1-AP

  Immunohistochemical analysis of paraffin-embedded human brown disease slide using 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brown disease using 27105-1-AP

  Immunohistochemical analysis of paraffin-embedded human brown disease slide using 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 27105-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27105-1-AP (Cytokeratin 8 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 27105-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27105-1-AP (Cytokeratin 8 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 27105-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 27105-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:4000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 27105-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 27105-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:4000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 27105-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Cytokeratin 8-specific antibody (27105-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IHC detected in: human tonsillitis tissue, human colon tissue, mouse skin tissue, rat skin tissue, human brown disease; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 2 publications below

Product Information

27105-1-AP targets Cytokeratin 8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cytokeratin 8 fusion protein Ag25844
• Full Name: keratin 8
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC008200
• Gene Symbol: Cytokeratin 8
• Gene ID (NCBI): 3856
• RRID: AB_2918117
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo.

Publications

Species	Application	Title
human	WB	Transl Lung Cancer Res; Identification of lysosomal genes associated with prognosis in lung adenocarcinoma; Authors - Houqiang Li
human	IHC	Environ Toxicol; Multidimensional progressive single-cell sequencing reveals cell microenvironment composition and cancer heterogeneity in lung cancer; Authors - Hua Chen
human	WB,IHC	J Cancer; Identification and validation of a novel apoptosis-related prognostic risk score model for lung adenocarcinoma; Authors - Yan Wang