Validation Data Gallery
|Positive WB detected in
|HeLa cells, HepG2 cells
|Positive IHC detected in
|human tonsillitis tissue, human colon tissue, mouse skin tissue, rat skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:2000-1:16000
|IHC : 1:2000-1:8000
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
The immunogen of 27105-1-AP is Cytokeratin 8-specific Fusion Protein expressed in E. coli.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|Cytokeratin 8-specific fusion protein Ag25844
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo.
|Product Specific Protocols
|WB protocol for Cytokeratin 8-specific antibody 27105-1-AP
|IHC protocol for Cytokeratin 8-specific antibody 27105-1-AP
|IF protocol for Cytokeratin 8-specific antibody 27105-1-AP
|Click here to view our Standard Protocols
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